Assay name​
HS_POU5F1_1491639 QuantiNova LNA PCR Assay
Wet-lab validated​
dPCR wet-lab validated
Assay specification​
Best coverage assay for POU5F1
Best transcript assay for ENST00000412166
Assay targets 3 transcripts
Assay is within same exon
IMPORTANT: May detect gDNA
Pre-designed assay for dPCR and qPCR. Wet-lab validated for dPCR.
Best transcript assay for ENST00000412166
Assay targets 3 transcripts
Assay is within same exon
IMPORTANT: May detect gDNA
Pre-designed assay for dPCR and qPCR. Wet-lab validated for dPCR.
Gene symbol​
POU5F1
Species​
Homo sapiens
Main target​
ENST00000412166
Exons numbers​
3
Ensembl exons ids​
ENSE00003580282
Amplicon start​
806
Amplicon length​
69
Assay targets​
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENST00000412166
Protein coding
NM_001285987;NM_001173531;NM_001285986;NM_203289
Chr.CHR_HSCHR6_MHC_DBB_CTG1: 31158060 - 31160001
ENST00000434616
Protein coding
Chr.CHR_HSCHR6_MHC_DBB_CTG1: 31158060 - 31164434
ENST00000463773
Processed transcript
Chr.CHR_HSCHR6_MHC_DBB_CTG1: 31158060 - 31159017