Assay name​
HS_CD274_1671395 QuantiNova LNA PCR Assay
Wet-lab validated​
qPCR and dPCR wet-lab validated
Assay specification​
Best coverage assay for CD274
Assay targets 4 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR and dPCR.
Assay in Focus Panel
Assay targets 4 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR and dPCR.
Assay in Focus Panel
Gene symbol​
CD274
Gene aliases​
B7-H;B7H1;PD-L1;PDL1;B7-H1;PDCD1LG1
Species​
Homo sapiens
Main target​
ENST00000498261
Exons numbers​
3-5
Ensembl exons ids​
ENSE00001884494;ENSE00001859182;ENSE00001940080
Amplicon start​
511
Amplicon length​
101
Assay targets​
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENST00000381573
Protein coding
CCDS59118
NM_001267706
Chr.9: 5450503 - 5470566
ENST00000381577
Protein coding
CCDS6464
NM_014143
Chr.9: 5450542 - 5470554
ENST00000492923
Processed transcript
Chr.9: 5462935 - 5465912
ENST00000498261
Processed transcript
Chr.9: 5456114 - 5467866