Assay name
MM_SIRT1_2284981 QuantiNova LNA PCR Assay
Wet-lab validated
qPCR wet-lab validated
Assay specification
Best coverage assay for Sirt1
Assay targets 6 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Assay targets 6 transcripts
Assay spans exon
Pre-designed assay for dPCR and qPCR. Wet-lab validated for qPCR.
Assay in Focus Panel
Gene symbol
Sirt1
Species
Mus musculus
Main target
ENSMUST00000020257
Exons numbers
6-7
Ensembl exons ids
ENSMUSE00000453122;ENSMUSE00001213633
Amplicon start
1172
Amplicon length
92
Assay targets
Transcript Ensembl Id
Bio Type
Ccds
Ref Seq
Chromosome Location
ENSMUST00000020257
Protein coding
CCDS23898
NM_019812
Chr.10: 63319007 - 63339032
ENSMUST00000105442
Protein coding
CCDS48585
NM_001159589
Chr.10: 63319058 - 63339035
ENSMUST00000120239
Protein coding
CCDS23898
Chr.10: 63319005 - 63381704
ENSMUST00000148516
Retained intron
Chr.10: 63322147 - 63325626
ENSMUST00000177694
Protein coding
Chr.10: 63319007 - 63339032