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GeneGlobe ID: PAHS-133Z | Cat. No.: 330231 | RT2 Profiler PCR Arrays

RT² Profiler™ PCR Array Human Liver Cancer

RT2 Profiler PCR Array

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Product Specification

Target List

Up-Regulated in Hepatocellular Carcinoma
BCL2, BIRC5, CCND1, CFLAR (CASPER), FZD7, IGF2, IRS1, LEF1, MCL1, MET, NFKB1, PIN1, PTK2 (FAK), RAC1, RHOA, SMAD7, STAT3, TCF4, TGFA, TGFB1, TNFSF10 (TRAIL), XIAP
Down-Regulated in Hepatocellular Carcinoma
BAX, BID, CASP8 (FLICE), CDKN1A (p21CIP1, WAF1), CDKN2A (P16INK4A), DLC1, FADD, FAS, HHIP, IGFBP1, IGFBP3, ITGB1, PTEN, RB1, SMAD4 (MADH4), SOCS1, SOCS3, TGFBR2, TNFRSF10B (DR5)
Signal Transduction
Classical WNT Signaling​
CCND1, CDKN2A (P16INK4A), CTNNB1, FZD7, LEF1, MTDH, PIN1, RB1, SFRP2, TCF4
TGFβ Signaling​
SMAD4 (MADH4), SMAD7, TGFB1, TGFBR2
AKT & PI3 Kinase Signaling​
AKT1, IRS1, PTEN
IGF / IGFR Signaling​
IGF2, IGFBP1, IGFBP3, IRS1
Hippo Signaling​
YAP1
RAS / RAF / MAP Kinase Signaling​
CDKN1A (p21CIP1, WAF1), CDKN2A (P16INK4A), DLC1, HRAS, NRAS, RAC1, RASSF1, RB1, RHOA, TP53 (p53)
EGFR Signaling​
ADAM17 (CD156B), CDH13, EGF, EGFR (ERBB1), TGFA, TGFB1
MET / HGF Signaling​
MET, HGF
G-Protein Signaling​
CDH13, CDKN1A (p21CIP1, WAF1), CDKN2A (P16INK4A), DAB2IP, DLC1, HRAS, NRAS, RAC1, RASSF1, RB1, RELN, RHOA, TP53 (p53)
Epithelial-to-Mesenchymal Transition (EMT)
CTNNB1, HGF, LEF1, SMAD4 (MADH4), SMAD7, TGFB1
Cell Cycle
BIRC5, CCND1, CCND2, CDKN1A (p21CIP1, WAF1), CDKN1B (P27KIP1), CDKN2A (P16INK4A), E2F1, EP300, GADD45B, IGF2, MYC, PTEN, RASSF1, RUNX3
Apoptosis
BAX, BCL2, BCL2L1 (BCLXL), BCL2L1 (BCLXL), BCL2L1 (BCLXL), BID, BIRC2 (c-IAP1), BIRC5, CASP8 (FLICE), CFLAR (CASPER), DLC1, E2F1, EP300, FADD, FAS, FHIT, GADD45B, GSTP1, MCL1, MSH2, PYCARD (TMS1, ASC), RUNX3, SOCS3, TERT, TNFRSF10B (DR5), TNFSF10 (TRAIL), WT1
Immune & Inflammatory Responses
CCL5 (RANTES), CXCR4, NFKB1, PTGS2 (COX2), RAC1, TGFB1, TLR4
Extracellular Matrix (ECM) & Cell Adhesion Molecules
ADAM17 (CD156B), AKT1, CDH1 (E-Cadherin), CDH13, CFLAR (CASPER), DLC1, EGFR (ERBB1), EP300, HGF, ITGB1, LEF1, OPCML (OBCAM), PTEN, PYCARD (TMS1, ASC), RAC1, RELN, RHOA, SMAD7, SOCS1, SOCS3, TGFB1
Angiogenesis
ANGPT2, CASP8 (FLICE), CDH13, CTNNB1, EGF, FLT1 (VEGFR1), KDR (VEGFR3), PDGFRA, PTEN, PTK2 (FAK), TGFBR2, VEGFA
DNA Damage & Repair
CCND1, CDKN1A (p21CIP1, WAF1), MSH2, MSH3, TP53 (p53), XIAP

Product Resources

Array Layout for PCR Plates
File Size: 139.26 KBLanguage: English
Array Layout for Rotor-Gene Q
File Size: 133.7 KBLanguage: English

Resources

Supplementary Protocols (10)
Bio-Rad CFX96 and CFX384 instrument setup instructions for RT2 Profiler PCR Arrays
PDF (298KB)
Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (1MB)
Eppendorf Mastercycler ep realplex instrument setup instructions for RT2 Profiler PCR Arrays
PDF (554KB)
ABI 7900HT (for SDS Software 2.1, 2.3 and 2.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (320KB)
Stratagene Mx3000P qPCR System real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (259KB)
Rotor-Gene Q real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (175KB)
ABI 7500 & ABI 7500 FAST (Software Version 2.0.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (388KB)
Bio-Rad iCycler & iQ Real-Time PCR Systems (for Software Version 3.1) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (249KB)
ABI StepOnePlus (for Software Version 2.0) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (77KB)
Life Technologies ViiA7 (ViiA 7 Software v1.2) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (511KB)
Brochures & Guides (3)
Technical Guide to QIAGEN PCR Arrays
PDF (2MB)
RT2 Profiler PCR Arrays: Pathway Analysis - (EN)
PDF (1MB)
Total RNA Discovery - (EN)
PDF (255KB)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Safety Data Sheets (1)
Safety Data Sheets
Articles (17)
(EN)Managing mortality: how cells regulate telomerase activity
(EN) The role of inflammation in cancer
(EN)A good transcription factor gone rogue: the two faces of NRF2 in oxidative stress
(EN)Metabolic homeostasis: regulation of FOXO by class IIa HDACs
(EN) Chemokines: manipulators of metastasis
(EN) Cellular stress, TGF-β, and EMT: what are the true origins of fibrosis?
(EN) Regulation of BAX mediated apoptosis by BCL2 family members
(EN)Inflammation and neurodegenerative disease: a role for the estrogen receptor in suppression of inflammation in the brain
(EN) Control of PPARγ and mTOR in insulin resistance
(EN) What enhances the inflammatory response: new players and unexpected roles
(EN) DNA damage repair — regulation through autophagy
(EN) Lipid autophagy in obesity and atherosclerosis: chewing the fat
(EN) Induced pluripotent stem cells: real stem cells?
(EN) Silencing cell-cell communication to combat drug toxicity
(EN) miRNA regulation of angiogenesis: new roles for IGF-1R signaling and heparin
(EN) Master Regulators Direct TGF-beta and Smad Signaling in Different Cell Types
(EN)Regulation of the extracellular matrix by miRNA: impact on cancer metastases
Download Files (3)
RT2 Profiler PCR Array Data Analysis v3.5 Handbook
PDF (770KB)
For analyzing gene expression data from RT2 Profiler PCR Arrays 
Housekeeping Gene Data Analysis
XLSX (60KB)
Data analysis file for RT² Profiler PCR Array Housekeeping Genes
Catalog number- 330231
Pathway number- PAXX-000
RNA QC Data Analysis
XLS (484KB)

Data analysis file for RT² ProfilerRT² Profiler™ PCR Array RT2 RNA QC
Catalog number- 330231
Pathway number- PAXX-999

Scientific Posters (1)
Explore the RNA Universe!
PDF (1MB)
Poster for download
Kit Handbooks (1)
RT2 Profiler PCR Array Handbook
PDF (702KB)
For pathway-focused gene expression profiling using real-time RT-PCR
Performance Data (7)
RT2 Profiler Housekeeping Genes PCR Array Data Analysis 1808
XLSX (65KB)
PCR_Array_4x96_384-Well_Conversion Spreadsheet
XLSX (38KB)
RT2 Profiler PCR Array Data Analysis Spreadsheet 1904
XLSX (3MB)
RT2 Profiler RNA QC PCR Array Data Analysis Spreadsheet 1808
XLSX (60KB)
Universal Custom PCR Array Conversion
XLSX (291KB)
RT2 qPCR Assay Data Analysis 1808 Spreadsheet
XLSX (104KB)
RT2 Profiler PCR Array 384HT Data Analysis Spreadsheet 1808
XLSX (1MB)
Instrument Technical Documents (2)
QIAGEN Service Core - (EN)
PDF (333KB)
For gene expression and genomic analysis
(EN) - RT2 Profiler PCR Arrays
PDF (431KB)
For pathway-focused gene expression analysis
White Papers (2)
RT2 Profiler PCR Arrays: Pathway-focused gene expression profiling with qRT-PCR
PDF (1MB)
RT2 Profiler PCR Array application examples
PDF (563KB)
Gene Expression Analysis (1)
(EN) Wnt Signaling: GSK3 Inhibition Through Sequestration in Multivesicular Bodies
Certificates of Analysis (1)
Certificates of Analysis
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Custom Products

You’ve prepared your RNA-seq libraries and finally sequenced them. Have you hit a roadblock with downstream data analysis? It doesn’t have to be that way.

RT2 Profiler PCR Arrays Tailored to Your Requirements

You’ve prepared your RNA-seq libraries and finally sequenced them. Have you hit a roadblock with downstream data analysis? It doesn’t have to be that way.

Consultation & Product Management

You’ve prepared your RNA-seq libraries and finally sequenced them. Have you hit a roadblock with downstream data analysis? It doesn’t have to be that way.

Frequently Purchased Together

The list of products below has to include products you need to prepare the experiment. Make sure you have all the required products while adding to the cart.
Product pictureProduct picture
CAT NO./ID 330401
RT2 First Strand Kit (12)
For twelve 20 µl first strand cDNA synthesis reactions; Buffer GE (24 µl), 5x Buffer BC3 (48 µl), RE3 Reverse Transcriptase Mix (24 µl), Control P2 (12 µl), Nuclease-Free Water (1 ml)
$334.00
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CAT NO./ID 330404
RT2 First Strand Kit (50)
For fifty 20 µl first strand cDNA synthesis reactions; Buffer GE (100 µl), 5x Buffer BC3 (200 µl), RE3 Reverse Transcriptase Mix (100 µl), Control P2 (50 µl), Nuclease-Free Water (1 ml)
$681.00
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CAT NO./ID 330500
RT² SYBR Green qPCR Mastermix (2)
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Arrays or 200 x 25 µl reactions or 400 x 10 µl reactions
$282.00
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CAT NO./ID 330503
RT² SYBR Green qPCR Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
$2,265.00
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CAT NO./ID 330520
RT² SYBR Green ROX qPCR Mastermix (2)
Contains 2 x 1.35 ml tubes: for 2 x 96-well RT² PCR Arrays, 1 x 384-well RT² PCR Array or 200 x 25 µl reactions or 400 x 10 µl reactions
$279.00
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CAT NO./ID 330521
RT² SYBR Green ROX qPCR Mastermix (8)
Contains 8 x 1.35 ml tubes: for 8 x 96-well RT² PCR Arrays, 4 x 384-well RT² PCR Arrays or 800 x 25 µl reactions or 2000 x 10 µl reactions
$1,065.00
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CAT NO./ID 330522
RT² SYBR Green ROX qPCR Mastermix (12)
Contains 12 x 1.35 ml tubes: for 12 x 96-well RT² PCR Arrays, 8 x 384-well RT² PCR Arrays or 1200 x 25 µl reactions or 3000 x 10 µl reactions
$1,518.00
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CAT NO./ID 330523
RT² SYBR Green ROX qPCR Mastermix (24)
Contains 24 x 1.35 ml tubes: for 24 x 96-well RT² PCR Arrays, 16 x 384-well RT² PCR Arrays or 2400 x 25 µl reactions or 6000 x 10 µl reactions
$2,276.00
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CAT NO./ID 217004
miRNeasy Mini Kit (50)
For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers
$506.00
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CAT NO./ID 338162
RT2 PCR Array Loading Reservoir (12)
12 x 5 ml capacity, irradiation-sterilized RT2 PCR Array Loading Reservoirs
$33.50
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CAT NO./ID 74104
RNeasy Mini Kit (50)
50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers
$432.00
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CAT NO./ID 79254
RNase-Free DNase Set (50)
1500 Kunitz units RNase-free DNase I, RNase-free Buffer RDD, and RNase-free water for 50 RNA minipreps
$154.00
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