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Positive results with as little as 25 ng RNA.
High reproducibility among different users.
PCR arrays yield highly accurate results.
A single gene-specific product in every reaction.
Simple procedure.

RT² Profiler™ PCR Array Pig mTOR Signaling

GeneGlobe ID: PASS-098Z | Cat. No.: 330231 | RT2 Profiler PCR Arrays

Product Specification

RT2 Profiler PCR Array

Target List

mTOR Complexes
mTORC1 Complex
MLST8MTORRPTOR
mTORC2 Complex
MLST8MTORRICTOR
mTOR Upstream Regulators
mTORC1 Positive Regulation
AKT1AKT2AKT3IGF1IKBKBINSINSRIRS1KRASMAPK1MAPK1MAPK3MAPK3NRASPDPK1PIK3C3PIK3CALOC100622663PIK3CDPIK3CGPIK3R2PLD1PLD2RHEBRPS6KA1RPS6KA2RPS6KA4LOC100152046RRAGARRAGCRRAGDTELO2
mTORC2 Positive Regulation
AKT1AKT2AKT3MAPK1MAPK1MAPK3MAPK3PDPK1PIK3C3PIK3CALOC100622663PIK3CDPIK3CGPIK3R2RHEBRPS6KA1RPS6KA2RPS6KA4LOC100152046
mTORC1 Negative Regulation
AKT1S1CAB39CAB39LDDIT4DDIT4LDEPTORFKBP1AFKBP8IGFBP3PRKAA1PRKAA2PRKAB1PRKAB2PRKAG1LOC100738310PTENSTK11STRADBTP53TSC1TSC2YWHAQ
mTORC2 Negative Regulation
CAB39CAB39LDDIT4DDIT4LDEPTORPRKAA1PRKAA2PRKAB1PRKAB2PRKAG1LOC100738310STK11STRADBTSC1TSC2
mTOR Downstream Effectors
mTORC1 Positive Regulation
CHUKEIF4EHIF1AIKBKBRPS6RPS6KB1RPS6KB2TP53VEGFAVEGFBVEGFC
mTORC2 Positive Regulation
AKT1CDC42GSK3BHSPA4ILKMYO1CPRKCAPRKCBPRKCEPRKCGRHOARPS6KB1SGK1
mTORC1 Negative Regulation
EIF4EBP1EIF4EBP2PPP2CAPPP2R2BPPP2R4TP53ULK1ULK2
Cellular Processes
Amino Acid Response
PIK3C3RRAGARRAGCRRAGD
Angiogenesis
CHUKDDIT4DDIT4LHIF1AIKBKBVEGFAVEGFBVEGFC
Autophagy
PIK3C3ULK1ULK2
Cytoskeletal Organization
CDC42PRKCAPRKCBPRKCEPRKCGRHOA
Growth Factor Response
BRAFIGF1IGFBP3KRASMAPK1MAPK1MAPK3MAPK3NRASRPS6KA1RPS6KA2RPS6KA4LOC100152046
Energy Stress
PRKAA1PRKAA2PRKAB1PRKAB2PRKAG1LOC100738310
Insulin Signaling
AKT1AKT2AKT3INSINSRIRS1PDPK1PIK3CALOC100622663PIK3CDPIK3CGPIK3R2TP53
Lipid Metabolism
BRAFPLD1PLD2
Translation
EIF4EEIF4EBP1EIF4EBP2PPP2CAPPP2R2BPPP2R4RPS6RPS6KA1RPS6KA2RPS6KA4LOC100152046RPS6KB1RPS6KB2

Product Resources

icon_0041_download-s Save Target List
icon_0245_cc_gen_pdf-s Array Layout for PCR Plates
File Size: 141.62 KBLanguage: English
icon_0245_cc_gen_pdf-s Array Layout for Rotor-Gene Q
File Size: 135.66 KBLanguage: English

Resources

Supplementary Protocols (10)
Bio-Rad CFX96 and CFX384 instrument setup instructions for RT2 Profiler PCR Arrays
PDF (298KB)
Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (1MB)
Eppendorf Mastercycler ep realplex instrument setup instructions for RT2 Profiler PCR Arrays
PDF (554KB)
ABI 7900HT (for SDS Software 2.1, 2.3 and 2.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (320KB)
Stratagene Mx3000P qPCR System real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (259KB)
Rotor-Gene Q real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (175KB)
ABI 7500 & ABI 7500 FAST (Software Version 2.0.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (388KB)
Bio-Rad iCycler & iQ Real-Time PCR Systems (for Software Version 3.1) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (249KB)
ABI StepOnePlus (for Software Version 2.0) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (77KB)
Life Technologies ViiA7 (ViiA 7 Software v1.2) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (511KB)
Brochures & Guides (3)
Technical Guide to QIAGEN PCR Arrays
PDF (2MB)
RT2 Profiler PCR Arrays: Pathway Analysis - (EN)
PDF (1MB)
Total RNA Discovery - (EN)
HTML (255KB)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Safety Data Sheets (1)
Safety Data Sheets
Articles (17)
(EN)Managing mortality: how cells regulate telomerase activity
(EN) The role of inflammation in cancer
(EN)A good transcription factor gone rogue: the two faces of NRF2 in oxidative stress
(EN)Metabolic homeostasis: regulation of FOXO by class IIa HDACs
(EN) Chemokines: manipulators of metastasis
(EN) Cellular stress, TGF-β, and EMT: what are the true origins of fibrosis?
(EN) Regulation of BAX mediated apoptosis by BCL2 family members
(EN)Inflammation and neurodegenerative disease: a role for the estrogen receptor in suppression of inflammation in the brain
(EN) Control of PPARγ and mTOR in insulin resistance
(EN) What enhances the inflammatory response: new players and unexpected roles
(EN) DNA damage repair — regulation through autophagy
(EN) Lipid autophagy in obesity and atherosclerosis: chewing the fat
(EN) Induced pluripotent stem cells: real stem cells?
(EN) Silencing cell-cell communication to combat drug toxicity
(EN) miRNA regulation of angiogenesis: new roles for IGF-1R signaling and heparin
(EN) Master Regulators Direct TGF-beta and Smad Signaling in Different Cell Types
(EN)Regulation of the extracellular matrix by miRNA: impact on cancer metastases
Download Files (3)
RT2 Profiler PCR Array Data Analysis v3.5 Handbook
PDF (770KB)
For analyzing gene expression data from RT2 Profiler PCR Arrays 
Housekeeping Gene Data Analysis
XLSX (60KB)
Data analysis file for RT² Profiler PCR Array Housekeeping Genes
Catalog number- 330231
Pathway number- PAXX-000
RNA QC Data Analysis
XLS (484KB)

Data analysis file for RT² ProfilerRT² Profiler™ PCR Array RT2 RNA QC
Catalog number- 330231
Pathway number- PAXX-999

Scientific Posters (1)
Explore the RNA Universe!
PDF (1MB)
Poster for download
Kit Handbooks (1)
RT2 Profiler PCR Array Handbook
PDF (702KB)
For pathway-focused gene expression profiling using real-time RT-PCR
Performance Data (7)
RT2 Profiler Housekeeping Genes PCR Array Data Analysis 1808
XLSX (65KB)
PCR_Array_4x96_384-Well_Conversion Spreadsheet
XLSX (38KB)
RT2 Profiler PCR Array Data Analysis Spreadsheet 1904
XLSX (3MB)
RT2 Profiler RNA QC PCR Array Data Analysis Spreadsheet 1808
XLSX (60KB)
Universal Custom PCR Array Conversion
XLSX (291KB)
RT2 qPCR Assay Data Analysis 1808 Spreadsheet
XLSX (104KB)
RT2 Profiler PCR Array 384HT Data Analysis Spreadsheet 1808
XLSX (1MB)
Instrument Technical Documents (2)
QIAGEN Service Core - (EN)
PDF (333KB)
For gene expression and genomic analysis
(EN) - RT2 Profiler PCR Arrays
PDF (431KB)
For pathway-focused gene expression analysis
White Papers (2)
RT2 Profiler PCR Arrays: Pathway-focused gene expression profiling with qRT-PCR
PDF (1MB)
RT2 Profiler PCR Array application examples
PDF (563KB)
Gene Expression Analysis (1)
(EN) Wnt Signaling: GSK3 Inhibition Through Sequestration in Multivesicular Bodies
Certificates of Analysis (1)
Certificates of Analysis
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