The dPCR Microbial DNA Detection Assays are highly suited for the detection of bacterial, fungal and viral species and microbial antibiotic resistance or virulence factor genes. A variety of samples can be analyzed, including stool, sputum, vaginal swab, sewage and others.

Additionally, the Custom dPCR Microbial assays design tool allows to seamlessly design assays for any microbial (bacteria, fungi, viruses) target of interest not covered by our predesigned dPCR Microbial DNA Detection Assays.

dPCR wet-lab tested 5-plex bundles

Bundle ID	Application Field	Assay Catalog Numbers and Fluorophore*	Targets (NCBI Taxonomy ID)
WW-001	Wastewater 1	DMA00278-F DMA00291-R DMA00340-H DMA00192-T DMA00344-C	Pseudomonas aeruginosa (287) Salmonella enterica (28901) Vibrio cholerae (666) Legionella pneumophila (446) Yersinia enterocolitica (630)
WW-002	Wastewater 2	DMA00340-H DMA00344-R DMA00199-C DMA00192-T DMA00710-F	Vibrio cholerae (666) Yersinia enterocolitica (630) Listeria monocytogenes (1639) Legionella pneumophila (446) Human corona virus SARS-CoV-2 (2697049)
WW-003	Wastewater 3	DMA00109-F DMA00192-H DMA00340-T DMA00194-R DMA00344-C	Clostridium perfringens (1502) Legionella pneumophila (446) Vibrio cholerae (666) Leptospira alexanderi (100053) Yersinia enterocolitica (630)
HM-001	Human Microbiome 1	DMA00148-F DMA00143-T DMA00024-R DMA00003-C DMA00150-H	Faecalibacterium prausnitzii (853) Eubacterium rectale (39491) Akkermansia muciniphila (239935) Acidaminococcus fermentans (951) Finegoldia magna (1260)
PB-001	Probiotics 1	DMA00177-F DMA00185-T DMA00061-C DMA00137-R DMA00320-H	Lactobacillus acidophilus (1579) Lactiplantibacillus plantarum (1590) Bifidobacterium bifidum (1681) Enterococcus faecium (1352) Streptococcus salivarius (1304)
RG-001	Resistance Genes 1	DMA00566-F DMA00542-H DMA00576-T DMA00574-R DMA00575-C	Fluoroquinolone resistance gene QnrS Class D beta-lactamase OXA-10 group Vancomycin resistance gene vanB Tetracycline efflux pump gene tetA Tetracycline efflux pump gene tetB
RG-002	Resistance Genes 2	DMA00557-T DMA00528-R DMA00548-F DMA00587-H DMA00553-C	Fluoroquinolone resistance geneQepA Class B beta-lactamase blaVIM-1 group Class D beta-lactamase OXA-48 group Sulfonamide resistance gene sul1 (43904) Class D beta-lactamase OXA-58 group
VG-001	Virulence Genes 1	DMA00614-F DMA00635-T DMA00677-H DMA00680-R DMA00597-C	Minor fimbrial subunit (fimH) Gamma-hemolysin component B (hlgB) Shiga-like toxin 1 subunit B encoded within prophage CP-933V Shiga toxin subunit B; receptor binding subunit Accessory cholera enterotoxin (ace)
CP-001	Cannabis Production 1	DMA00278-F DMA00302-H DMA00365-R DMA00199-C	Pseudomonas aeruginosa (287) Staphylococcus aureus (1280) Aspergillus niger (5061) Listeria monocytogenes (1639)
HM-002	Human Microbiome 2	DMA00271-F DMA00017-T DMA00049-C DMA00142-H DMA00317-R	Prevotella oralis (28134) Actinomyces viscosus (1656) Bacteroides fragilis (817) Eubacterium infirmum (56774) Streptococcus oralis (1303)
RG-003	Resistance Genes 3	DMA00579-F DMA00577-T DMA00573-R DMA00502-H DMA00559-C	aac(6')-Ib vanC oprm QnrB-1 group CTX-M-1 Group
VG-002	Virulence Genes 2	DMA00664-F DMA00677-H DMA00678-T DMA00642-R DMA00680-C	ply stx1B stx2A invA stxB
VG-003	Virulence Genes 3	DMA00688-F DMA00668-H DMA00596-T DMA00597-R DMA00611-C	wbkA ptxA ace (E. faecalis) ace (V. cholerae) efaA

* F: FAM, H: HEX, T: TAMRA, R: ROX or C: Cy5

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