Product Name
dPCR CNV Probe Gene of Interest for MLH1
GeneGlobe Cat.No. (Assay ID)
DCG0000005
Assay type
Gene of Interest
Species
Human (Homo sapiens)
Gene symbol
MLH1
Ensembl Gene ID
ENSG00000076242
NCBI Gene Id
4292
Strand
Binds to reverse (3’ to 5’) bottom genome strand
Amplicon length
71
Amplicon region
CCCCTTTGGAGGGACTGCCTATCTTCATTCTTCGACTAGCCACTGAGGTCAGTGATCAAGCAGATACTAAGCATTTCGGTACATGCATGTGTGCTGGAGGG
Recommended Reference Assay
TERT (DCR0000186), AP3B1 (DCR0000238), RPP30 (DCR0000181), AGO1 (DCR0000536)
Recommended Restriction Enzyme
AluI, HaeIII, EcoRI, XbaI, PvuII
Wet-lab validated
dPCR wet-lab validated
Hydrolysis Probe
FAM, ATTO 550, Cy5
Primer Purification
Desalted
Probe Purification
HPLC