Product Name
dPCR CNV Probe Gene of Interest for EGFR
GeneGlobe Cat.No. (Assay ID)
DCG0000180
Assay type
Gene of Interest
Species
Human (Homo sapiens)
Gene symbol
EGFR
Ensembl Gene ID
ENSG00000146648
NCBI Gene Id
1956
Strand
Binds to forward (5’ to 3’) top genome strand
Amplicon length
141
Amplicon region
GGCAGCAGAGCTCCTGCTCTTCTTTGTCCTCATATACGAGCACCTCTGGACTTAAAACTTGAGGAACTGGATGGAGAAAAGTTAATGGTCAGCAGCGGGTTACATCTTCTTTCATGCGCCTTTCCATTCTTTGGATCAGTAGTCACTAACGTTCGCCAGCCATAAGTCCTC
Recommended Reference Assay
TERT (DCR0000186), AP3B1 (DCR0000238), RPP30 (DCR0000181), AGO1 (DCR0000536)
Recommended Restriction Enzyme
CviQI, AluI, HaeIII, EcoRI, XbaI, PvuII
Wet-lab validated
dPCR wet-lab validated
Hydrolysis Probe
FAM, ATTO 550, Cy5
Primer Purification
Desalted
Probe Purification
HPLC