dPCR CNV Probe Gene of Interest for WT1

Product Specification

Gene symbol: WT1
Ensembl Gene ID: ENSG00000184937
dPCR wet-lab validated

Product Name

dPCR CNV Probe Gene of Interest for WT1

GeneGlobe Cat.No. (Assay ID)

DCG0000309

Assay type

Gene of Interest

Species

Human (Homo sapiens)

Gene symbol

WT1

Ensembl Gene ID

ENSG00000184937

NCBI Gene Id

7490

Strand

Binds to reverse (3’ to 5’) bottom genome strand

Amplicon length

Amplicon region

AAATGTCCAGCCATAGGCGGTACAGTTAGTATGATGATCATCAACACTTGGCAAGATGTGAGTCTACCTGTGGACACTTTACGATGTAGACCAAATCTTAG

Recommended Reference Assay

TERT (DCR0000186), AP3B1 (DCR0000238), RPP30 (DCR0000181), AGO1 (DCR0000536)

Recommended Restriction Enzyme

AluI, HaeIII, EcoRI, XbaI, PvuII

Wet-lab validated

dPCR wet-lab validated

Hydrolysis Probe

FAM, ATTO550, Cy5, ATTO700

Primer Purification

Desalted

Probe Purification

HPLC

Reaction size

300rxns, 500rxns and 1000rxns depending on selected dye

Product Resources

Validation Data

File Size: 226.97 KBLanguage: English

Resources

Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.

For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System

Introducing the dPCR CNV Probe Gene of Interest for WT1, a cutting-edge addition to our dPCR CNV Probe Assays lineup, specifically designed for Digital PCR applications. This premium product offers unparalleled performance for researchers working with Human models. Leveraging advanced technology, the dPCR CNV Probe Gene of Interest for WT1 facilitates accurate and reliable results, making it an indispensable tool for your scientific investigations. Whether you're conducting Digital PCR, or any other sophisticated analyses, this product from our dPCR CNV Probe Assays collection ensures optimal efficiency and reproducibility for Human studies. Embrace the future of research with dPCR CNV Probe Gene of Interest for WT1 and elevate your work to new heights.

Country and Language

dPCR CNV Probe Gene of Interest for WT1

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Product Specification

Product Resources

Resources

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