GeneGlobe ID: DMA00043 | Cat. No.: 250207 | dPCR Microbial DNA Detection Assays
dPCR Microbial DNA Detection Assay for Bacillus subtilis
Product Specification
Bacterial detection assay targeting the 16S ribosomal RNA gene
dPCR wet-lab validated
dPCR wet-lab validated
Product name
Bacillus subtilis
GeneGlobe Cat.No. (Assay ID)
DMA00043
Target type
Microbial Id
Target region
16S ribosomal RNA gene
Target (NCBI taxonomy ID)
Bacillus subtilis (1423)
Bacillus natto
Bacillus uniflagellatus
Vibrio subtilis
Bacillus natto
Bacillus uniflagellatus
Vibrio subtilis
Template accession
AB110598.1
Taxonomy
Bacteria
Secondary targets/specificity
Bacillus velezensis (492670)
Bacillus amyloliquefaciens subsp. plantarum
Bacillus methylotrophicus Madhaiyan et al. 2010 emend. Dunlap et al. 2015
Bacillus methylotrophicus subsp. plantarum
Bacillus methylotrophicus
Bacillus oryzicola
Bacillus tequilensis (227866)
Bacillus stercoris (2054641)
Bacillus subtilis subsp. stecoris
Bacillus amyloliquefaciens (1390)
Bacillus amyloliquefaciens subsp. amyloliquefaciens
Bacillus amyloliquifaciens
Bacillus amyloliquefaciens subsp. plantarum
Bacillus methylotrophicus Madhaiyan et al. 2010 emend. Dunlap et al. 2015
Bacillus methylotrophicus subsp. plantarum
Bacillus methylotrophicus
Bacillus oryzicola
Bacillus tequilensis (227866)
Bacillus stercoris (2054641)
Bacillus subtilis subsp. stecoris
Bacillus amyloliquefaciens (1390)
Bacillus amyloliquefaciens subsp. amyloliquefaciens
Bacillus amyloliquifaciens
Application field
Probiotics
Gastrointestinal Infections
Gastrointestinal Infections
Wet-lab tested in singleplex
Yes, tested dye - HEX
Wet-lab tested in multiplex
No
Recommended restriction enzyme
EcoRI
Template present in Microbial DNA Positive Control
Yes
Region of Interest
CGAGCGGACAGATGGGAGCTTGCTCCCTGATGTTAGCGGCGGACGGGTGAGTAACACGTGGGTAACCTGCCTGTAAGACTGGGATAACTCCGGGAAACCGGGGCTAATACCGGATGGTTGTTTGAACCGCATGGTTCAAACATAAAAGGTGGCTTCGGCTACCACTTACAGATGGACCCGCGGCGCATTAGCTAGTTG
Reaction size
200rxns
Product Resources
File Size: 238.74 KBLanguage: English
Resources
Available Product Catalog (2)
Quick-Start Protocols (1)
Application Notes (2)
Technical Information (1)
Kit Handbooks (1)
Scientific Posters (1)
Brochures & Guides (2)
Safety Data Sheets (1)
Certificates of Analysis (1)
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