Virulence gene detection assay targeting the bacterial shiga toxin Stx1a subunit B gene
dPCR wet-lab validated
GeneGlobe Cat.No. (Assay ID)
DMA00677
Target region
Bacterial shiga toxin Stx1a subunit B gene
Target (NCBI taxonomy ID)
shiga-like toxin 1 subunit B encoded within prophage CP-933V
Template accession
EF441595.1
Application field
Microbial Virulence
Wet-lab tested in singleplex
Yes, tested dye - HEX
Wet-lab tested in multiplex
Yes
Recommended restriction enzyme
EcoRI
Template present in Microbial DNA Positive Control
Yes
Region of Interest
TGGCGACGCCTGATTGTGTAACTGGAAAGGTGGAGTATACAAAATATAATGATGACGATACCTTTACAGTTAAAGTGGGTGATAAAGAATTATTTACCAACAGATGGAATCTTCAGTCTCTTCTTCTCAGTGCGCAAATTACGGGGATGACTGTAACCATTAAAACTAATGCCTGTCATAATGGAGGGGGATTCAGCGAAG