Functionally verified siRNA directed against Human PRKRA, (NM_001139517, NM_001139518, NM_003690).
NM_001139518

NM_003690

NM_001139517

Species
Human (Homo sapiens)
Official Name
protein kinase, interferon-inducible double stranded RNA dependent activator
Detected Transcripts
NM_001139518 (1627 bp), NM_003690 (1826 bp), NM_001139517 (1906 bp)
Potential Off - Target mRNAs, 3' UTR Seed Match
222484|NM_153371|AS // 51426|NM_016218|S // 84181|NM_032221|S // 256536|NM_174937|S // 285753|NM_001083535|S // 285753|NM_173830|S
Current Target mRNAs
8575|NM_001139518 // 8575|NM_003690 // 8575|NM_001139517
Experimental Details
72% Knockdown
Cell Line: HeLa
23
Concentration of transfected siRNA: 25 nM
Transfection reagent:
HiPerFect Transfection ReagentTransfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against
GAPDH, Cyclophilin