Functionally verified siRNA directed against Human PRKG2, (NM_006259).
NM_006259
Name​
Hs_PRKG2_5
Species​
Human (Homo sapiens)
Official Name​
protein kinase, cGMP-dependent, type II
Official Symbol​
PRKG2
Entrez Gene ID​
5593
Detected Transcripts​
NM_006259 (4822 bp)
Potential Off-Target mRNAs, High Homology​
390058|NM_001004750|642|AS // 140838|NM_152667|2485|S
Current Target mRNAs​
5593|NM_006259
Experimental Details​
78% Knockdown
Cell Line: HEK 293
23
Concentration of transfected siRNA: 25 nM
Transfection reagent: RNAiFect Transfection Reagent
Transfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Cell Line: HEK 293
23
Concentration of transfected siRNA: 25 nM
Transfection reagent: RNAiFect Transfection Reagent
Transfection format: 96 well
Quantification of gene silencing: 48 hours post-transfection
RT-PCR type: One-step
Normalization Against​
GAPDH, Cyclophilin