GeneGlobe ID: PAHS-098Z | Cat. No.: 330231 | RT2 Profiler PCR Arrays

RT² Profiler™ PCR Array Human mTOR Signaling

RT2 Profiler PCR Array
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Product Specification

Target List
mTOR Complexes
mTORC1 Complex​
MLST8, MTOR, RPTOR
mTORC2 Complex​
MLST8, MAPKAP1 (SIN1), MTOR, RICTOR
mTOR Upstream Regulators
mTORC1 Positive Regulation​
AKT1, AKT2, AKT3, HRAS, IGF1, IKBKB (IKKβ), INS, INSR, IRS1, MAPK1 (ERK2), MAPK3 (ERK1), PDPK1, PIK3C3 (VPS34), PIK3CA (p110-alpha), PIK3CB, PIK3CD, PIK3CG, PLD1, PLD2, RHEB, RPS6KA1, RPS6KA2, RPS6KA5, RRAGA, RRAGB, RRAGC, RRAGD, TELO2
mTORC2 Positive Regulation​
AKT1, AKT2, AKT3, MAPK1 (ERK2), MAPK3 (ERK1), PDPK1, PIK3C3 (VPS34), PIK3CA (p110-alpha), PIK3CB, PIK3CD, PIK3CG, RHEB, RPS6KA1, RPS6KA2, RPS6KA5
mTORC1 Negative Regulation​
AKT1S1 (PRAS40), CAB39, CAB39L, DDIT4 (REDD1), DDIT4L (REDD2), DEPTOR, FKBP1A (FKBP12), FKBP8, IGFBP3, PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3, PTEN, STK11 (LKB1), STRADB, TP53 (p53), TSC1, TSC2, YWHAQ (14-3-3θ)
mTORC2 Negative Regulation​
CAB39, CAB39L, DDIT4 (REDD1), DDIT4L (REDD2), DEPTOR, PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3, STK11 (LKB1), STRADB, TSC1, TSC2
mTOR Downstream Effectors
mTORC1 Positive Regulation​
CHUK (IKKα), EIF4B, EIF4E, HIF1A, IKBKB (IKKβ), RPS6, RPS6KB1, RPS6KB2, TP53 (p53), VEGFA, VEGFB, VEGFC
mTORC2 Positive Regulation​
AKT1, CDC42, GSK3B, HSPA4 (HSP70), ILK, MYO1C, PRKCA, PRKCB, PRKCE, PRKCG, RHOA, RPS6KB1, SGK1
mTORC1 Negative Regulation​
EIF4EBP1 (4E-BP1), EIF4EBP2, PPP2CA, PPP2R2B, PTPA, TP53 (p53), ULK1, ULK2
Cellular Processes Regulated by mTOR Signaling
Amino Acid Response​
PIK3C3 (VPS34), RRAGA, RRAGB, RRAGC, RRAGD
Angiogenesis​
CHUK (IKKα), DDIT4 (REDD1), DDIT4L (REDD2), HIF1A, IKBKB (IKKβ), VEGFA, VEGFB, VEGFC
Autophagy​
PIK3C3 (VPS34), ULK1, ULK2
Cytoskeletal Organization​
CDC42, PRKCA, PRKCB, PRKCE, PRKCG, RHOA
Growth Factor Response​
HRAS, IGF1, IGFBP3, MAPK1 (ERK2), MAPK3 (ERK1), RPS6KA1, RPS6KA2, RPS6KA5
Energy Stress​
PRKAA1 (AMPK), PRKAA2, PRKAB1, PRKAB2, PRKAG1, PRKAG2, PRKAG3
Insulin Signaling​
AKT1, AKT2, AKT3, INS, INSR, IRS1, PDPK1, PIK3CA (p110-alpha), PIK3CB, PIK3CD, PIK3CG, TP53 (p53)
Lipid Metabolism​
PLD1, PLD2
Translation​
EIF4B, EIF4E, EIF4EBP1 (4E-BP1), EIF4EBP2, PPP2CA, PPP2R2B, PTPA, RPS6, RPS6KA1, RPS6KA2, RPS6KA5, RPS6KB1, RPS6KB2
Gene Resource List
Array Layout for PCR Plates
File Size: 138.39 KBLanguage: English
Array Layout for Rotor-Gene Q
File Size: 132.85 KBLanguage: English

Resources

Brochures & Guides (3)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Download Files (3)
For analyzing gene expression data from RT2 Profiler PCR Arrays 
Data analysis file for RT² Profiler PCR Array Housekeeping Genes
Catalog number- 330231
Pathway number- PAXX-000
RNA QC Data Analysis
XLS (484KB)

Data analysis file for RT² ProfilerRT² Profiler™ PCR Array RT2 RNA QC
Catalog number- 330231
Pathway number- PAXX-999

Scientific Posters (1)
Poster for download
Kit Handbooks (1)
For pathway-focused gene expression profiling using real-time RT-PCR
Instrument Technical Documents (2)
For gene expression and genomic analysis
For pathway-focused gene expression analysis
Certificates of Analysis (1)
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