Spef1 Gene Summary [Mouse]
Enables microtubule binding activity. Involved in axonemal central apparatus assembly; cilium movement; and negative regulation of microtubule depolymerization. Located in 9+2 motile cilium; axonemal central apparatus; and ciliary tip. Is expressed in several structures, including central nervous system; genitourinary system; gut; respiratory system; and sensory organ. Orthologous to human SPEF1 (sperm flagellar 1). [provided by Alliance of Genome Resources, Jul 2025]
Details
| Type | Protein Coding |
| Official Symbol | Spef1 |
| Official Name | sperm flagellar 1 [Source:MGI Symbol;Acc:MGI:3513546] |
| Ensembl ID | ENSMUSG00000027329 |
| Bio databases IDs | |
| Aliases | sperm flagellar 1 |
| Synonyms | 4931426K16Rik, C20orf28, CLAMP, RGD1311267, SPEF1A, sperm flagellar 1 |
| Species | Mouse, Mus musculus |
| Orthologies |
Protein Domains
A protein domain is a distinct structural or functional region within a protein that can evolve, function, and exist independently of the rest of the protein chain. These domains in mouse Spef1 often fold into stable, three-dimensional structures and are associated with specific biological functions, such as binding to DNA, other proteins, or small molecules.
- CH-like domain in sperm protein
- microtubule binding
- actin binding
- calponin homology (CH) domain superfamily
- protein binding
Top Findings
The most significant associations for this gene, including commonly observed domains, pathway involvement, and functional highlights based on current data.
Subcellular Expression
Locations within the cell where the protein is known or predicted to be active, providing insight into its function and cellular context.
- Cytoplasm
- motile cilia
- ciliary tip
- microtubules
- axonemes
- apical membrane
- basolateral membrane
- microvilli
- filopodia
- lamellipodia
Gene Ontology Annotations
Describes the biological processes, cellular components, and molecular functions associated with the mouse Spef1 gene, providing context for its role in the cell.
Biological Process
Functions and activities the gene product is involved in
- microtubule bundle formation
- regulation of cytoskeleton organization
- regulation of Wnt receptor signaling pathway, planar cell polarity pathway
- negative regulation of microtubule depolymerization
- lamellipodium assembly
- cilium movement
- filopodium assembly
- cell migration
Cellular Component
Where in the cell the gene product is active
- microvillus
- cytoplasm
- stress fiber
- filopodium
- basolateral plasma membrane
- axoneme
- apical plasma membrane
- lamellipodium
- microtubule
Molecular Function
What the gene product does at the molecular level
- protein binding
- actin binding
- microtubule binding