Lig4 Gene Summary [Mouse]
Enables ATP binding activity and DNA ligase (ATP) activity. Involved in double-strand break repair via nonhomologous end joining. Acts upstream of or within several processes, including hemopoiesis; response to gamma radiation; and somatic diversification of immune receptors via germline recombination within a single locus. Part of DNA-dependent protein kinase-DNA ligase 4 complex. Is expressed in liver and metanephros. Used to study DNA ligase IV deficiency. Human ortholog(s) of this gene implicated in several diseases, including DNA ligase IV deficiency; colorectal cancer; multiple myeloma; pancreatic cancer; and prostate cancer. Orthologous to human LIG4 (DNA ligase 4). [provided by Alliance of Genome Resources, Jul 2025]
Details
| Type | Protein Coding |
| Official Symbol | Lig4 |
| Official Name | ligase IV, DNA, ATP-dependent [Source:MGI Symbol;Acc:MGI:1335098] |
| Ensembl ID | ENSMUSG00000049717 |
| Bio databases IDs | |
| Aliases | ligase IV, DNA, ATP-dependent |
| Synonyms | 5830471N16Rik, DNA ligase 4, LIG4S, ligase IV, DNA, ATP-dependent, tiny |
| Species | Mouse, Mus musculus |
| Orthologies |
Protein Domains
A protein domain is a distinct structural or functional region within a protein that can evolve, function, and exist independently of the rest of the protein chain. These domains in mouse Lig4 often fold into stable, three-dimensional structures and are associated with specific biological functions, such as binding to DNA, other proteins, or small molecules.
- BRCT
- catalytic domain
- DNA ligase N terminus
- DNA ligase
- BRCT domain, a BRCA1 C-terminus domain
- DNA ligase I, ATP-dependent (dnl1)
- ATP binding
- DNA ligase (ATP)
- enzyme
- protein binding
- magnesium ion binding
- DNA binding
- ATP dependent DNA ligase domain
- AMP binding
- Adenylation domain of proteins similar to ATP-dependent polynucleotide ligases
- ligase
- BRCA1 C Terminus (BRCT) domain
- DNA ligase IV
- DNA ligase oligonucleotide-binding fold domain
- breast cancer carboxy-terminal domain
Pathways
Biological processes and signaling networks where the Lig4 gene in mouse plays a role, providing insight into its function and relevance in health or disease.
Top Findings
The most significant associations for this gene, including commonly observed domains, pathway involvement, and functional highlights based on current data.
disease
- neoplasia
- cancer
- Lig4 syndrome
- hereditary disorder
- microcephaly
- sporadic amyotrophic lateral sclerosis
- lymphomagenesis
- hematologic cancer
- lymphoid cancer
- hematological neoplasia
phenotypes
- abnormal B cell activation
- abnormal B cell differentiation
- abnormal DNA repair
- abnormal T cell activation
- abnormal T cell differentiation
- abnormal T cell receptor V(D)J recombination
- abnormal T cell receptor beta chain V(D)J recombination
- abnormal brainstem morphology
- abnormal cell physiology
- abnormal diencephalon morphology
- abnormal immune system cell morphology
- abnormal immune system morphology
- abnormal immune system organ morphology
- abnormal immune system physiology
- abnormal lymphocyte cell number
- abnormal lymphopoiesis
- abnormal proerythroblast morphology
- abnormal regulatory T cell morphology
- abnormal striatum morphology
- abnormal thymus morphology
- absent CD8-positive alpha-beta T cells
- arrested B cell differentiation
- arrested T cell differentiation
- chromosomal instability
- chromosome breakage
- decreased B cell number
- decreased CD4-positive alpha beta T cell number
- decreased CD8-positive alpha-beta T cell number
- decreased CD8-positive naive alpha-beta T cell number
- decreased IgA level
- decreased IgD level
- decreased IgG level
- decreased IgG2b level
- decreased IgG3 level
- decreased IgM level
- decreased T cell proliferation
- decreased body size
- decreased body weight
- decreased bone marrow cell number
- decreased cell proliferation
- decreased double-negative T cell number
- decreased double-positive T cell number
- decreased fetal size
- decreased fibroblast proliferation
- decreased hematopoietic stem cell number
- decreased immunoglobulin level
- decreased interferon-gamma secretion
- decreased interleukin-13 secretion
- decreased lymphocyte cell number
- decreased spleen weight
- decreased splenocyte number
- decreased thymocyte number
- decreased thymus weight
- impaired humoral immune response
- increased CD8-positive alpha-beta T cell number
- increased NK cell number
- increased T cell apoptosis
- increased T cell derived lymphoma incidence
- increased anti-chromatin antibody level
- increased anti-double stranded DNA antibody level
- increased cellular sensitivity to gamma-irradiation
- increased cellular sensitivity to ionizing radiation
- increased central memory CD8 positive alpha-beta T cell number
- increased colon adenocarcinoma incidence
- increased effector memory CD4-positive alpha-beta T cell number
- increased forebrain apoptosis
- increased medulloblastoma incidence
- increased neuron apoptosis
- increased spinal cord apoptosis
- induced chromosome breakage
- intestinal inflammation
- lethality throughout fetal growth and development complete penetrance
- lethality throughout fetal growth and development incomplete penetrance
- liver inflammation
- postnatal growth retardation
- premature death
- prenatal lethality complete penetrance
- prenatal lethality incomplete penetrance
- reduced fertility
- small spleen
- small thymus
- spontaneous chromosome breakage
- thin cerebral cortex
role in cell
- accumulation in
- cell death
- proliferation
- growth
- apoptosis
- differentiation
- number
- function
- development
- abnormal morphology
Subcellular Expression
Locations within the cell where the protein is known or predicted to be active, providing insight into its function and cellular context.
- Nucleus
- nuclear fraction
- soluble fraction
- intracellular membrane-bounded organelle
- nuclear foci
- chromosome
- nucleoplasm
Gene Ontology Annotations
Describes the biological processes, cellular components, and molecular functions associated with the mouse Lig4 gene, providing context for its role in the cell.
Biological Process
Functions and activities the gene product is involved in
- cell proliferation
- positive regulation of chromosome organization
- response to X-ray
- DNA ligation
- neurogenesis
- double-strand break repair via nonhomologous end joining
- double-strand break repair
- negative regulation of neuron apoptotic process
- cell division
- DNA ligation involved in DNA repair
- fibroblast proliferation
- DNA ligation involved in DNA recombination
- in utero embryonic development
- positive regulation of fibroblast proliferation
- stem cell proliferation
- DNA biosynthetic process
- somatic stem cell maintenance
- cellular response to ionizing radiation
- response to gamma radiation
- neuron apoptotic process
- isotype switching
- V(D)J recombination
- T cell differentiation in thymus
- T cell receptor V(D)J recombination
- immunoglobulin V(D)J recombination
- nucleotide-excision repair, DNA gap filling
- single strand break repair
- cellular response to lithium ion
- central nervous system development
- pro-B cell differentiation
- chromosome organization
- positive regulation of neurogenesis
Cellular Component
Where in the cell the gene product is active
- condensed chromosome
- chromosome, telomeric region
- nucleus
- nonhomologous end joining complex
- DNA-dependent protein kinase-DNA ligase 4 complex
- DNA ligase IV complex
- intracellular membrane-bounded organelle
- nucleoplasm
Molecular Function
What the gene product does at the molecular level
- magnesium ion binding
- ATP binding
- DNA binding
- DNA ligase (ATP) activity
- protein binding
- DNA ligase activity
- ligase activity