Dhfr Gene Summary [Mouse]
Enables dihydrofolate reductase activity and folate reductase activity. Involved in several processes, including positive regulation of nitric-oxide synthase activity; tetrahydrobiopterin biosynthetic process; and tetrahydrofolate metabolic process. Acts upstream of or within dTMP biosynthetic process. Located in mitochondrion. Is active in cytosol and nucleus. Is expressed in several structures, including genitourinary system; hemolymphoid system; integumental system; limb; and nervous system. Human ortholog(s) of this gene implicated in acute lymphoblastic leukemia. Orthologous to several human genes including DHFR (dihydrofolate reductase). [provided by Alliance of Genome Resources, Apr 2025]
Details
| Type | Protein Coding |
| Official Symbol | Dhfr |
| Official Name | dihydrofolate reductase [Source:MGI Symbol;Acc:MGI:94890] |
| Ensembl ID | ENSMUSG00000021707 |
| Bio databases IDs | |
| Aliases | dihydrofolate reductase |
| Synonyms | 8430436I03RIK, DHFR1, DHFR4, DHFRP1, dihydrofolate reductase, dihydrofolic acid reductase, DYR |
| Species | Mouse, Mus musculus |
| Orthologies |
Protein Domains
A protein domain is a distinct structural or functional region within a protein that can evolve, function, and exist independently of the rest of the protein chain. These domains in mouse Dhfr often fold into stable, three-dimensional structures and are associated with specific biological functions, such as binding to DNA, other proteins, or small molecules.
- NADPH binding
- dihydrofolate reductase
- binding protein
- folic acid binding
- NADP binding
- mRNA binding
- DHFR
- nucleic acid binding translation repressor
- CH-NH group:NAD or NADP oxidoreductase
- enzyme
- folate/MTX binding site
- NADPH binding domain
Pathways
Biological processes and signaling networks where the Dhfr gene in mouse plays a role, providing insight into its function and relevance in health or disease.
Top Findings
The most significant associations for this gene, including commonly observed domains, pathway involvement, and functional highlights based on current data.
disease
- epithelial cancer
- epithelial neoplasia
- cancer
- preeclampsia
- acute lymphoblastic leukemia
- ovarian cancer
- acute myeloid leukemia
- myelodysplastic syndrome
- malignant neoplasm
- nasopharyngeal cancer
regulates
- folic acid
- dihydrofolate
- NOS
- DHFR
- MIR24
- sapropterin
- pemetrexed
- L-tetrahydrofolic acid
- methotrexate
- purine
role in cell
- cell death
- apoptosis
- quantity
- growth
- translation in
- formation
- colony formation
- expansion
- number
- transformation
Subcellular Expression
Locations within the cell where the protein is known or predicted to be active, providing insight into its function and cellular context.
- Nucleus
- Cytoplasm
- Mitochondria
- cytosol
- mitochondrial matrix
- mitochondrial inner membrane
Gene Ontology Annotations
Describes the biological processes, cellular components, and molecular functions associated with the mouse Dhfr gene, providing context for its role in the cell.
Biological Process
Functions and activities the gene product is involved in
- response to methotrexate
- tetrahydrobiopterin biosynthetic process
- positive regulation of nitric-oxide synthase activity
- dihydrofolate metabolic process
- folic acid metabolic process
- tetrahydrofolate metabolic process
- regulation of removal of superoxide radicals
- tetrahydrofolate biosynthetic process
- negative regulation of translation
- one-carbon metabolic process
- axon regeneration
Cellular Component
Where in the cell the gene product is active
- mitochondrion
- cytosol
Molecular Function
What the gene product does at the molecular level
- NADP binding
- translation repressor activity, nucleic acid binding
- NADPH binding
- mRNA binding
- dihydrofolate reductase activity
- folic acid binding