Gene Globe

If you’re interested in broad transcript detection and want to know whether the gene of interest is expressed in your sample, the assay marked as “Best coverage” is your best choice. This gene-covering assay is designed to capture most biologically relevant transcripts of the gene, making it ideal for general screening or when you don’t need to differentiate between isoforms.

If you want to focus on a specific transcript, choose the assay labeled “Best transcript assay.” This option is optimized to detect the transcript of interest with high specificity, targeting the most relevant isoforms of that transcript. It’s well-suited for pathway analysis or targeted expression studies where precise quantification of one transcript matters.

For experiments that require isoform-level resolution, such as studying alternative splicing, look for the assay marked “Best transcript-specific assay.” This one is splice variant–specific, meaning it’s designed to detect only a single isoform. Use this when you need to differentiate between specific isoforms of a transcript.

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