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dPCR Probe Assays für Kopienzahlvariation (CNV)

Für die lokusspezifische Analyse von Kopienzahlvariationen und -veränderungen mittels Einzelplex- oder Multiplex-dPCR

Mehr als 200 im Wet-Lab validierte Assays und ein Tool für die kundenspezifische In-silico-Assayentwicklung
Farbstoffauswahl ermöglicht das Multiplexing mit bis zu 5 Zielen je Reaktion
Zielgen-, Referenz- und Zentromerreferenzassays verfügbar
Gebrauchsfertige Assays wurden für viele verschiedene Proben wie FFPE, Zelllinien und gDNA validiert
Einfacher und schneller dPCR-Workflow auf dem QIAcuity
Komfortable Auswertung der Kopienzahldaten mit der QIAcuity Software Suite

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Accessories

The list of products below has to include products you need to prepare the experiment. Make sure you have all the required products while adding to the cart.

QIAcuity Probe PCR Kit (1 ml)

Cat. No.: 250101

1 ml 4x concentrated QIAcuity Probe Mastermix, 2 x 1.9 ml Water

QIAcuity Probe PCR Kit (5 mL)

Cat. No.: 250102

5 x 1 mL 4x concentrated QIAcuity Probe Mastermix, 8 x 1.9 mL Water

QIAcuity Probe PCR Kit (25 ml)

Cat. No.: 250103

5 x 5 ml 4x concentrated QIAcuity Probe Mastermix, 4 x 20 ml Water

QIAcuity EG PCR Kit (1 ml)

Cat. No.: 250111

1 ml 3x concentrated QIAcuity EvaGreen Mastermix, 1 x 1.9 ml Water

DNeasy Blood & Tissue Kit (250)

Cat. No.: 69506

250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

RNeasy Mini Kit (250)

Cat. No.: 74106

250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers.

Resources

For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System

Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.

Download Safety Data Sheets for QIAGEN product components.

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dPCR Probe Assays für Kopienzahlvariation (CNV)

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