dPCR Copy Number Variation (CNV) Probe Assays

For locus-specific analysis of copy number variations and alterations by singleplex or multiplex dPCR

More than 200 wet-lab-validated assays and a custom tool for in-silico assay design
Dye selection enables multiplexing of up to 5 targets per reaction
Gene of interest, reference and centromeric reference assays available
Ready-to-use assays have been validated for a range of samples, such as FFPE, cell lines and gDNA
Simple and fast dPCR workflow on the QIAcuity
Convenient copy number data analysis using the QIAcuity Software Suite

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Accessories

The list of products below has to include products you need to prepare the experiment. Make sure you have all the required products while adding to the cart.

QIAcuity Probe PCR Kit (1 ml)

Cat. No.: 250101

1 ml 4x concentrated QIAcuity Probe Mastermix, 2 x 1.9 ml Water

QIAcuity Probe PCR Kit (5 mL)

Cat. No.: 250102

5 x 1 mL 4x concentrated QIAcuity Probe Mastermix, 8 x 1.9 mL Water

QIAcuity Probe PCR Kit (25 ml)

Cat. No.: 250103

5 x 5 ml 4x concentrated QIAcuity Probe Mastermix, 4 x 20 ml Water

QIAcuity EG PCR Kit (1 ml)

Cat. No.: 250111

1 ml 3x concentrated QIAcuity EvaGreen Mastermix, 1 x 1.9 ml Water

DNeasy Blood & Tissue Kit (250)

Cat. No.: 69506

250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)

RNeasy Mini Kit (250)

Cat. No.: 74106

250 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), RNase-free Reagents and Buffers.

Resources

Here, we present a workflow that combines two technologies, cellenONE and QIAcuity Digital PCR, which accelerate and streamline high-throughput analyses of target copy numbers in cultured cells. The workflow starts with detecting and sorting defined populations of cells as well as individual cells using cellenONE, followed by multiplexing dPCR on the QIAcuity platform. Copy number variations of target regions are then analyzed using the QIAcuity Software Suite, providing an intuitive and fast interpretation of results.

For locus-specific copy number variation (CNV) analysis using the QIAcuity Digital PCR System

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dPCR Copy Number Variation (CNV) Probe Assays

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