dPCR LNA Mutation Assays
For detecting specific DNA sequence mutations related to cancer and oncogenesis
- Duplex assay design detects mutated and wild-type sequences
- Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
- LNA-enhanced primers and probes increase assay specificity and sensitivity
- Wet-lab tested dPCR assays available for more than 200 targets
- Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
- Intended for use with QIAcuity MasterMix
ProductList_Title
dPCR Mutation Assay ABL1 12573 Human
Product_GeneGlobeID: DMH0000573 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E274K
Nucleotide Mutation: c.820G>A
dPCR wet-lab validated
Nucleotide Mutation: c.820G>A
dPCR wet-lab validated
dPCR Mutation Assay ABL1 12574 Human
Product_GeneGlobeID: DMH0000053 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E274V
Nucleotide Mutation: c.821A>T
dPCR wet-lab validated
Nucleotide Mutation: c.821A>T
dPCR wet-lab validated
dPCR Mutation Assay WT1 27370 Human
Product_GeneGlobeID: DMH0000048 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.D464N
Nucleotide Mutation: c.1390G>A
dPCR wet-lab validated
Nucleotide Mutation: c.1390G>A
dPCR wet-lab validated
dPCR Mutation Assay PIK3CA 12597 Human
Product_GeneGlobeID: DMH0000050 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G1049R
Nucleotide Mutation: c.3145G>C
dPCR wet-lab validated
Nucleotide Mutation: c.3145G>C
dPCR wet-lab validated
dPCR Mutation Assay PIK3CA 776 Human
Product_GeneGlobeID: DMH0000062 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.H1047L
Nucleotide Mutation: c.3140A>T
dPCR wet-lab validated
Nucleotide Mutation: c.3140A>T
dPCR wet-lab validated
dPCR Mutation Assay TP53 10891 Human
Product_GeneGlobeID: DMH0000104 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V272M
Nucleotide Mutation: c.814G>A
dPCR wet-lab validated
Nucleotide Mutation: c.814G>A
dPCR wet-lab validated
dPCR Mutation Assay PIK3CA 764 Human
Product_GeneGlobeID: DMH0000033 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E545G
Nucleotide Mutation: c.1634A>G
dPCR wet-lab validated
Nucleotide Mutation: c.1634A>G
dPCR wet-lab validated
dPCR Mutation Assay KRAS 1135364 Human
Product_GeneGlobeID: DMH0000024 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>C
dPCR wet-lab validated
Nucleotide Mutation: c.183A>C
dPCR wet-lab validated
dPCR Mutation Assay EGFR 6213 Human
Product_GeneGlobeID: DMH0000006 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L861Q
Nucleotide Mutation: c.2582T>A
dPCR wet-lab validated
Nucleotide Mutation: c.2582T>A
dPCR wet-lab validated
dPCR Mutation Assay NRAS 562 Human
Product_GeneGlobeID: DMH0000186 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G12C
Nucleotide Mutation: c.34G>T
dPCR wet-lab validated
Nucleotide Mutation: c.34G>T
dPCR wet-lab validated
dPCR Mutation Assay FLT3 303886 Human
Product_GeneGlobeID: DMH0000158 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.N676K
Nucleotide Mutation: c.2028C>A
dPCR wet-lab validated
Nucleotide Mutation: c.2028C>A
dPCR wet-lab validated
dPCR Mutation Assay FLT3 785 Human
Product_GeneGlobeID: DMH0000156 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.D835H
Nucleotide Mutation: c.2503G>C
dPCR wet-lab validated
Nucleotide Mutation: c.2503G>C
dPCR wet-lab validated
dPCR Mutation Assay TP53 43559 Human
Product_GeneGlobeID: DMH0000126 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V173L
Nucleotide Mutation: c.517G>T
dPCR wet-lab validated
Nucleotide Mutation: c.517G>T
dPCR wet-lab validated
dPCR Mutation Assay PTEN 5154 Human
Product_GeneGlobeID: DMH0000295 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R233*
Nucleotide Mutation: c.697C>T
dPCR wet-lab validated
Nucleotide Mutation: c.697C>T
dPCR wet-lab validated
dPCR Mutation Assay EGFR 18427 Human
Product_GeneGlobeID: DMH0000274 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E746_P753delinsVS
Nucleotide Mutation: c.2237_2257delinsTCT
dPCR wet-lab validated
Nucleotide Mutation: c.2237_2257delinsTCT
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12728 Human
Product_GeneGlobeID: DMH0000250 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E746_T751del
Nucleotide Mutation: c.2236_2253del
dPCR wet-lab validated
Nucleotide Mutation: c.2236_2253del
dPCR wet-lab validated
dPCR Mutation Assay PIK3CA 12459 Human
Product_GeneGlobeID: DMH0000212 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Q546R
Nucleotide Mutation: c.1637A>G
dPCR wet-lab validated
Nucleotide Mutation: c.1637A>G
dPCR wet-lab validated
dPCR Mutation Assay TP53 10645 Human
Product_GeneGlobeID: DMH0000353 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.C176F
Nucleotide Mutation: c.527G>T
dPCR wet-lab validated
Nucleotide Mutation: c.527G>T
dPCR wet-lab validated
dPCR Mutation Assay TP53 11081 Human
Product_GeneGlobeID: DMH0000374 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G245C
Nucleotide Mutation: c.733G>T
dPCR wet-lab validated
Nucleotide Mutation: c.733G>T
dPCR wet-lab validated
dPCR Mutation Assay NRAS 570 Human
Product_GeneGlobeID: DMH0000342 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G13C
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
dPCR Mutation Assay KRAS 528 Human
Product_GeneGlobeID: DMH0000331 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G13S
Nucleotide Mutation: c.37G>A
dPCR wet-lab validated
Nucleotide Mutation: c.37G>A
dPCR wet-lab validated
dPCR Mutation Assay TP53 10810 Human
Product_GeneGlobeID: DMH0000471 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.C242F
Nucleotide Mutation: c.725G>T
dPCR wet-lab validated
Nucleotide Mutation: c.725G>T
dPCR wet-lab validated
dPCR Mutation Assay ESR1 94250 Human
Product_GeneGlobeID: DMH0001110 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.D538G
Nucleotide Mutation: c.1613A>G
dPCR wet-lab validated
Nucleotide Mutation: c.1613A>G
dPCR wet-lab validated
dPCR Mutation Assay PIK3CA 27502 Human
Product_GeneGlobeID: DMH0000542 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E81K
Nucleotide Mutation: c.241G>A
dPCR wet-lab validated
Nucleotide Mutation: c.241G>A
dPCR wet-lab validated
dPCR Mutation Assay KRAS 534 Human
Product_GeneGlobeID: DMH0000527 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G13V
Nucleotide Mutation: c.38G>T
dPCR wet-lab validated
Nucleotide Mutation: c.38G>T
dPCR wet-lab validated
dPCR Mutation Assay EGFR 4170221 Human
Product_GeneGlobeID: DMH0001098 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L747_A750delinsS
Nucleotide Mutation: c.2240_2248del
dPCR wet-lab validated
Nucleotide Mutation: c.2240_2248del
dPCR wet-lab validated
dPCR Mutation Assay EGFR 6210 Human
Product_GeneGlobeID: DMH0001097 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L747_T751delinsS
Nucleotide Mutation: c.2240_2251del
dPCR wet-lab validated
Nucleotide Mutation: c.2240_2251del
dPCR wet-lab validated
dPCR Mutation Assay ESR1 3621514 Human
Product_GeneGlobeID: DMH0001103 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.S463F
Nucleotide Mutation: c.1388C>T
dPCR wet-lab validated
Nucleotide Mutation: c.1388C>T
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12988 Human
Product_GeneGlobeID: DMH0000041 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E709K
Nucleotide Mutation: c.2125G>A
dPCR wet-lab validated
Nucleotide Mutation: c.2125G>A
dPCR wet-lab validated
dPCR Mutation Assay PIK3CA 766 Human
Product_GeneGlobeID: DMH0000037 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Q546K
Nucleotide Mutation: c.1636C>A
dPCR wet-lab validated
Nucleotide Mutation: c.1636C>A
dPCR wet-lab validated
dPCR Mutation Assay FLT3 116249 Human
Product_GeneGlobeID: DMH0000009 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V491L
Nucleotide Mutation: c.1471G>C
dPCR wet-lab validated
Nucleotide Mutation: c.1471G>C
dPCR wet-lab validated
dPCR Mutation Assay NRAS 563 Human
Product_GeneGlobeID: DMH0000188 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G12S
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated
dPCR Mutation Assay TP53 3675521 Human
Product_GeneGlobeID: DMH0000114 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R273L
Nucleotide Mutation: c.818G>T
dPCR wet-lab validated
Nucleotide Mutation: c.818G>T
dPCR wet-lab validated
dPCR Mutation Assay FLT3 28042 Human
Product_GeneGlobeID: DMH0000281 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.S451F
Nucleotide Mutation: c.1352C>T
dPCR wet-lab validated
Nucleotide Mutation: c.1352C>T
dPCR wet-lab validated
dPCR Mutation Assay MET 735 Human
Product_GeneGlobeID: DMH0000256 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.?
Nucleotide Mutation: c.3083-52_3083-51insCT
dPCR wet-lab validated
Nucleotide Mutation: c.3083-52_3083-51insCT
dPCR wet-lab validated
dPCR Mutation Assay TP53 1645467 Human
Product_GeneGlobeID: DMH0000364 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E286K
Nucleotide Mutation: c.856G>A
dPCR wet-lab validated
Nucleotide Mutation: c.856G>A
dPCR wet-lab validated
dPCR Mutation Assay TP53 43947 Human
Product_GeneGlobeID: DMH0000463 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Y205C
Nucleotide Mutation: c.614A>G
dPCR wet-lab validated
Nucleotide Mutation: c.614A>G
dPCR wet-lab validated
dPCR Mutation Assay ESR1 1074635 Human
Product_GeneGlobeID: DMH0001107 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Y537N
Nucleotide Mutation: c.1609T>A
dPCR wet-lab validated
Nucleotide Mutation: c.1609T>A
dPCR wet-lab validated
dPCR Mutation Assay EGFR 13556 Human
Product_GeneGlobeID: DMH0000078 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.S752_I759del
Nucleotide Mutation: c.2253_2276del
dPCR wet-lab validated
Nucleotide Mutation: c.2253_2276del
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12419 Human
Product_GeneGlobeID: DMH0000075 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L747_T751delinsQ
Nucleotide Mutation: c.2238_2252delinsGCA
dPCR wet-lab validated
Nucleotide Mutation: c.2238_2252delinsGCA
dPCR wet-lab validated
dPCR Mutation Assay BRAF 477 Human
Product_GeneGlobeID: DMH0000039 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V600D
Nucleotide Mutation: c.1799_1800delinsAT
dPCR wet-lab validated
Nucleotide Mutation: c.1799_1800delinsAT
dPCR wet-lab validated
dPCR Mutation Assay JAK2 12600 Human
Product_GeneGlobeID: DMH0000021 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V617F
Nucleotide Mutation: c.1849G>T
dPCR wet-lab validated
Nucleotide Mutation: c.1849G>T
dPCR wet-lab validated
dPCR Mutation Assay BRAF 474 Human
Product_GeneGlobeID: DMH0000002 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V600R
Nucleotide Mutation: c.1798_1799delinsAG
dPCR wet-lab validated
Nucleotide Mutation: c.1798_1799delinsAG
dPCR wet-lab validated
dPCR Mutation Assay TP53 129857 Human
Product_GeneGlobeID: DMH0000119 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.P152L
Nucleotide Mutation: c.455C>T
dPCR wet-lab validated
Nucleotide Mutation: c.455C>T
dPCR wet-lab validated
dPCR Mutation Assay PIK3CA 746 Human
Product_GeneGlobeID: DMH0000204 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R88Q
Nucleotide Mutation: c.263G>A
dPCR wet-lab validated
Nucleotide Mutation: c.263G>A
dPCR wet-lab validated
dPCR Mutation Assay FLT3 19522 Human
Product_GeneGlobeID: DMH0000174 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V592A
Nucleotide Mutation: c.1775T>C
dPCR wet-lab validated
Nucleotide Mutation: c.1775T>C
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12370 Human
Product_GeneGlobeID: DMH0000266 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L747_P753delinsS
Nucleotide Mutation: c.2240_2257del
dPCR wet-lab validated
Nucleotide Mutation: c.2240_2257del
dPCR wet-lab validated
dPCR Mutation Assay KRAS 520 Human
Product_GeneGlobeID: DMH0000285 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G12V
Nucleotide Mutation: c.35G>T
dPCR wet-lab validated
Nucleotide Mutation: c.35G>T
dPCR wet-lab validated
dPCR Mutation Assay EGFR 6225 Human
Product_GeneGlobeID: DMH0000240 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E746_A750del
Nucleotide Mutation: c.2236_2250del
dPCR wet-lab validated
Nucleotide Mutation: c.2236_2250del
dPCR wet-lab validated
dPCR Mutation Assay PTEN 5219 Human
Product_GeneGlobeID: DMH0000296 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R130G
Nucleotide Mutation: c.388C>G
dPCR wet-lab validated
Nucleotide Mutation: c.388C>G
dPCR wet-lab validated
dPCR Mutation Assay IDH1 28751 Human
Product_GeneGlobeID: DMH0000228 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R132V
Nucleotide Mutation: c.394_395delinsGT
dPCR wet-lab validated
Nucleotide Mutation: c.394_395delinsGT
dPCR wet-lab validated
dPCR Mutation Assay FLT3 788 Human
Product_GeneGlobeID: DMH0000496 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.D835E
Nucleotide Mutation: c.2505T>G
dPCR wet-lab validated
Nucleotide Mutation: c.2505T>G
dPCR wet-lab validated
dPCR Mutation Assay NRAS 571 Human
Product_GeneGlobeID: DMH0000510 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G13S
Nucleotide Mutation: c.37G>A
dPCR wet-lab validated
Nucleotide Mutation: c.37G>A
dPCR wet-lab validated
dPCR Mutation Assay WT1 21392 Human
Product_GeneGlobeID: DMH0000575 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.A382Vfs*4
Nucleotide Mutation: c.1141_1144dup
dPCR wet-lab validated
Nucleotide Mutation: c.1141_1144dup
dPCR wet-lab validated
dPCR Mutation Assay ESR1 9994994 Human
Product_GeneGlobeID: DMH0001102 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.S463C
Nucleotide Mutation: c.1388C>G
dPCR wet-lab validated
Nucleotide Mutation: c.1388C>G
dPCR wet-lab validated
dPCR Mutation Assay ESR1 4774826 Human
Product_GeneGlobeID: DMH0001105 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L536R
Nucleotide Mutation: c.1607T>G
dPCR wet-lab validated
Nucleotide Mutation: c.1607T>G
dPCR wet-lab validated
dPCR Mutation Assay CDKN2A 12743 Human
Product_GeneGlobeID: DMH0000566 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.P48L
Nucleotide Mutation: c.143C>T
dPCR wet-lab validated
Nucleotide Mutation: c.143C>T
dPCR wet-lab validated
dPCR Mutation Assay EGFR 6240 Human
Product_GeneGlobeID: DMH0000085 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.T790M
Nucleotide Mutation: c.2369C>T
dPCR wet-lab validated
Nucleotide Mutation: c.2369C>T
dPCR wet-lab validated
dPCR Mutation Assay EGFR 13552 Human
Product_GeneGlobeID: DMH0000079 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E746_T751delinsIP
Nucleotide Mutation: c.2235_2251delinsAATTC
dPCR wet-lab validated
Nucleotide Mutation: c.2235_2251delinsAATTC
dPCR wet-lab validated
dPCR Mutation Assay WT1 21434 Human
Product_GeneGlobeID: DMH0000058 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R462Q
Nucleotide Mutation: c.1385G>A
dPCR wet-lab validated
Nucleotide Mutation: c.1385G>A
dPCR wet-lab validated
dPCR Mutation Assay IDH2 41590 Human
Product_GeneGlobeID: DMH0000018 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R140Q
Nucleotide Mutation: c.419G>A
dPCR wet-lab validated
Nucleotide Mutation: c.419G>A
dPCR wet-lab validated
dPCR Mutation Assay BRAF 473 Human
Product_GeneGlobeID: DMH0000001 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V600K
Nucleotide Mutation: c.1798_1799delinsAA
dPCR wet-lab validated
Nucleotide Mutation: c.1798_1799delinsAA
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12387 Human
Product_GeneGlobeID: DMH0000269 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L747_P753delinsQ
Nucleotide Mutation: c.2239_2258delinsCA
dPCR wet-lab validated
Nucleotide Mutation: c.2239_2258delinsCA
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12678 Human
Product_GeneGlobeID: DMH0000258 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E746_T751delinsA
Nucleotide Mutation: c.2237_2251del
dPCR wet-lab validated
Nucleotide Mutation: c.2237_2251del
dPCR wet-lab validated
dPCR Mutation Assay KRAS 532 Human
Product_GeneGlobeID: DMH0000289 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G13D
Nucleotide Mutation: c.38G>A
dPCR wet-lab validated
Nucleotide Mutation: c.38G>A
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12371 Human
Product_GeneGlobeID: DMH0000397 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E709V
Nucleotide Mutation: c.2126A>T
dPCR wet-lab validated
Nucleotide Mutation: c.2126A>T
dPCR wet-lab validated
dPCR Mutation Assay TP53 342245 Human
Product_GeneGlobeID: DMH0000382 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G154V
Nucleotide Mutation: c.461G>T
dPCR wet-lab validated
Nucleotide Mutation: c.461G>T
dPCR wet-lab validated
dPCR Mutation Assay NRAS 585 Human
Product_GeneGlobeID: DMH0000349 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Q61H
Nucleotide Mutation: c.183A>T
dPCR wet-lab validated
Nucleotide Mutation: c.183A>T
dPCR wet-lab validated
dPCR Mutation Assay TP53 220778 Human
Product_GeneGlobeID: DMH0000475 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R158H
Nucleotide Mutation: c.473G>A
dPCR wet-lab validated
Nucleotide Mutation: c.473G>A
dPCR wet-lab validated
dPCR Mutation Assay ESR1 6843697 Human
Product_GeneGlobeID: DMH0001106 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L536H
Nucleotide Mutation: c.1607T>A
dPCR wet-lab validated
Nucleotide Mutation: c.1607T>A
dPCR wet-lab validated
dPCR Mutation Assay DNMT3A 87007 Human
Product_GeneGlobeID: DMH0000043 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.P904L
Nucleotide Mutation: c.2711C>T
dPCR wet-lab validated
Nucleotide Mutation: c.2711C>T
dPCR wet-lab validated
dPCR Mutation Assay EGFR 5945664 Human
Product_GeneGlobeID: DMH0000052 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.C797S
Nucleotide Mutation: c.2390G>C
dPCR wet-lab validated
Nucleotide Mutation: c.2390G>C
dPCR wet-lab validated
dPCR Mutation Assay TP53 10733 Human
Product_GeneGlobeID: DMH0000090 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Q192*
Nucleotide Mutation: c.574C>T
dPCR wet-lab validated
Nucleotide Mutation: c.574C>T
dPCR wet-lab validated
dPCR Mutation Assay BRAF 460 Human
Product_GeneGlobeID: DMH0000047 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G469A
Nucleotide Mutation: c.1406G>C
dPCR wet-lab validated
Nucleotide Mutation: c.1406G>C
dPCR wet-lab validated
dPCR Mutation Assay KRAS 527 Human
Product_GeneGlobeID: DMH0000195 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G13C
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
Nucleotide Mutation: c.37G>T
dPCR wet-lab validated
dPCR Mutation Assay TP53 99626 Human
Product_GeneGlobeID: DMH0000131 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.C238F
Nucleotide Mutation: c.713G>T
dPCR wet-lab validated
Nucleotide Mutation: c.713G>T
dPCR wet-lab validated
dPCR Mutation Assay NOTCH1 4163569 Human
Product_GeneGlobeID: DMH0000144 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.T311P
Nucleotide Mutation: c.931A>C
dPCR wet-lab validated
Nucleotide Mutation: c.931A>C
dPCR wet-lab validated
dPCR Mutation Assay KRAS 521 Human
Product_GeneGlobeID: DMH0000286 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G12D
Nucleotide Mutation: c.35G>A
dPCR wet-lab validated
Nucleotide Mutation: c.35G>A
dPCR wet-lab validated
dPCR Mutation Assay EGFR 13427 Human
Product_GeneGlobeID: DMH0000271 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E709A
Nucleotide Mutation: c.2126A>C
dPCR wet-lab validated
Nucleotide Mutation: c.2126A>C
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12382 Human
Product_GeneGlobeID: DMH0000304 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L747_A750delinsP
Nucleotide Mutation: c.2239_2248delinsC
dPCR wet-lab validated
Nucleotide Mutation: c.2239_2248delinsC
dPCR wet-lab validated
dPCR Mutation Assay TP53 10714 Human
Product_GeneGlobeID: DMH0000362 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R158L
Nucleotide Mutation: c.473G>T
dPCR wet-lab validated
Nucleotide Mutation: c.473G>T
dPCR wet-lab validated
dPCR Mutation Assay FLT3 28040 Human
Product_GeneGlobeID: DMH0000500 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.D324N
Nucleotide Mutation: c.970G>A
dPCR wet-lab validated
Nucleotide Mutation: c.970G>A
dPCR wet-lab validated
dPCR Mutation Assay TP53 10758 Human
Product_GeneGlobeID: DMH0000440 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Y220C
Nucleotide Mutation: c.659A>G
dPCR wet-lab validated
Nucleotide Mutation: c.659A>G
dPCR wet-lab validated
dPCR Mutation Assay EGFR 13550 Human
Product_GeneGlobeID: DMH0000070 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E746_A750delinsIP
Nucleotide Mutation: c.2235_2248delinsAATTC
dPCR wet-lab validated
Nucleotide Mutation: c.2235_2248delinsAATTC
dPCR wet-lab validated
dPCR Mutation Assay FLT3 783 Human
Product_GeneGlobeID: DMH0000152 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.D835Y
Nucleotide Mutation: c.2503G>T
dPCR wet-lab validated
Nucleotide Mutation: c.2503G>T
dPCR wet-lab validated
dPCR Mutation Assay NOTCH1 4775081 Human
Product_GeneGlobeID: DMH0000148 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.I1616N
Nucleotide Mutation: c.4847T>A
dPCR wet-lab validated
Nucleotide Mutation: c.4847T>A
dPCR wet-lab validated
dPCR Mutation Assay KRAS 1159597 Human
Product_GeneGlobeID: DMH0000290 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.Q61K
Nucleotide Mutation: c.181C>A
dPCR wet-lab validated
Nucleotide Mutation: c.181C>A
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12369 Human
Product_GeneGlobeID: DMH0000275 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.L747_T751del
Nucleotide Mutation: c.2240_2254del
dPCR wet-lab validated
Nucleotide Mutation: c.2240_2254del
dPCR wet-lab validated
dPCR Mutation Assay EGFR 12416 Human
Product_GeneGlobeID: DMH0000236 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E746_T751delinsVA
Nucleotide Mutation: c.2237_2253delinsTTGCT
dPCR wet-lab validated
Nucleotide Mutation: c.2237_2253delinsTTGCT
dPCR wet-lab validated
dPCR Mutation Assay PTEN 5152 Human
Product_GeneGlobeID: DMH0000294 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R130*
Nucleotide Mutation: c.388C>T
dPCR wet-lab validated
Nucleotide Mutation: c.388C>T
dPCR wet-lab validated
dPCR Mutation Assay IDH1 88208 Human
Product_GeneGlobeID: DMH0000230 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R100Q
Nucleotide Mutation: c.299G>A
dPCR wet-lab validated
Nucleotide Mutation: c.299G>A
dPCR wet-lab validated
dPCR Mutation Assay TP53 43629 Human
Product_GeneGlobeID: DMH0000377 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R249W
Nucleotide Mutation: c.745A>T
dPCR wet-lab validated
Nucleotide Mutation: c.745A>T
dPCR wet-lab validated
dPCR Mutation Assay TP53 10710 Human
Product_GeneGlobeID: DMH0000361 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.E298*
Nucleotide Mutation: c.892G>T
dPCR wet-lab validated
Nucleotide Mutation: c.892G>T
dPCR wet-lab validated
dPCR Mutation Assay ABL1 12560 Human
Product_GeneGlobeID: DMH0000306 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.T334I
Nucleotide Mutation: c.1001C>T
dPCR wet-lab validated
Nucleotide Mutation: c.1001C>T
dPCR wet-lab validated
dPCR Mutation Assay KIT 1314 Human
Product_GeneGlobeID: DMH0000515 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.D816V
Nucleotide Mutation: c.2447A>T
dPCR wet-lab validated
Nucleotide Mutation: c.2447A>T
dPCR wet-lab validated
dPCR Mutation Assay KRAS 517 Human
Product_GeneGlobeID: DMH0000519 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G12S
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated
Nucleotide Mutation: c.34G>A
dPCR wet-lab validated
dPCR Mutation Assay WT1 27307 Human
Product_GeneGlobeID: DMH0000060 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.S381*
Nucleotide Mutation: c.1142C>A
dPCR wet-lab validated
Nucleotide Mutation: c.1142C>A
dPCR wet-lab validated
dPCR Mutation Assay EGFR 6239 Human
Product_GeneGlobeID: DMH0000057 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.G719A
Nucleotide Mutation: c.2156G>C
dPCR wet-lab validated
Nucleotide Mutation: c.2156G>C
dPCR wet-lab validated
dPCR Mutation Assay IDH2 33732 Human
Product_GeneGlobeID: DMH0000016 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.R172M
Nucleotide Mutation: c.515G>T
dPCR wet-lab validated
Nucleotide Mutation: c.515G>T
dPCR wet-lab validated
dPCR Mutation Assay BRAF 475 Human
Product_GeneGlobeID: DMH0000003 | Product_CatNo: Product_GGId_CatNo_Varies | dPCR LNA Mutation Assays
AA Mutation: p.V600E
Nucleotide Mutation: c.1799_1800delinsAA
dPCR wet-lab validated
Nucleotide Mutation: c.1799_1800delinsAA
dPCR wet-lab validated
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Application Note: Optimized urine liquid biopsy workflow: From sample collection to cfDNA stabilization and purification, ready for digital PCR analysis
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
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