dPCR LNA Mutation Assays

For detecting specific DNA sequence mutations related to cancer and oncogenesis

  • Duplex assay design detects mutated and wild-type sequences
  • Choice of FAM + HEX or Atto 550 + ROX fluorescent dye combinations
  • LNA-enhanced primers and probes increase assay specificity and sensitivity
  • Wet-lab tested dPCR assays available for more than 200 targets
  • Suitable for use with circulating tumor DNA, liquid biopsy, FFPE and other tissue samples
  • Intended for use with QIAcuity Probe PCR Kits

Product

Product List5 products

GeneGlobe ID: DMH0000015 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay IDH1 28750 Human
AA Mutation: p.R132L
Nucleotide Mutation: c.395G>T
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000063 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay IDH1 28749 Human
AA Mutation: p.R132G
Nucleotide Mutation: c.394C>G
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000228 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay IDH1 28751 Human
AA Mutation: p.R132V
Nucleotide Mutation: c.394_395delinsGT
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000066 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay IDH1 28748 Human
AA Mutation: p.R132S
Nucleotide Mutation: c.394C>A
dPCR wet-lab validated
Product Specification
GeneGlobe ID: DMH0000230 | Cat. No.: | dPCR LNA Mutation Assays
dPCR Mutation Assay IDH1 88208 Human
AA Mutation: p.R100Q
Nucleotide Mutation: c.299G>A
dPCR wet-lab validated
Product Specification
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Resources

Instrument User Manuals (1)
Quick-Start Protocols (1)
Safety Data Sheets (1)
Application Notes (1)
Digital PCR (dPCR) is a powerful technique that detects and quantifies ultra-rare mutations in a high background of wild-type cfDNA down to 0.1% variant allele frequency. Here, we describe end-to-end manual and automated workflows that enable accurate detection and absolute quantification of ultra-rare PIK3CA variants in cfDNA using the QIAcuity Digital PCR System.
Certificates of Analysis (1)