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GeneGlobe ID: PARN-098Z | Cat. No.: 330231 | RT2 Profiler PCR Arrays

RT² Profiler™ PCR Array Rat mTOR Signaling

RT2 Profiler PCR Array

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Product Specification

Target List

mTOR Complexes
mTORC1 Complex​
MtorMlst8Rptor
mTORC2 Complex​
MtorMlst8Mapkap1 (Sin1)
mTOR Upstream Regulators
mTORC1 Positive Regulation​
Akt1Akt2Akt3Rps6ka4HrasIgf1Ikbkb (IKK2)Ins2InsrIrs1KrasMapk1 (Erk2)Mapk3 (ERK1)NrasPdpk1Pik3c3 (Vps34)Pik3ca (p110-alpha)Pik3cbPik3cdPik3cgPik3r1 (PI3KA)Pik3r2Pld1Pld2RhebRps6ka1Rps6ka2Rps6ka5RragaRragb (QuantiNova Symbol: LOC108348096)RragcRragd
mTORC2 Positive Regulation​
Akt1Akt2Akt3Rps6ka4Mapk1 (Erk2)Mapk3 (ERK1)Pdpk1Pik3c3 (Vps34)Pik3ca (p110-alpha)Pik3cbPik3cdPik3cgPik3r1 (PI3KA)Pik3r2RhebRps6ka1Rps6ka2Rps6ka5
mTORC1 Negative Regulation​
Akt1s1 (Pras40)Cab39Cab39lDdit4 (Redd1)Ddit4l2 (Redd2)Fkbp1a (Fkbp12)Fkbp8Igfbp3Prkaa1 (Ampk)Prkaa2Prkab1Prkab2Prkag1Prkag2Prkag3PtenStk11 (Lkb1)Tp53 (p53)Tsc1Tsc2Ywhaq (14-3-3θ)
mTORC2 Negative Regulation​
Cab39Cab39lDdit4 (Redd1)Ddit4l2 (Redd2)Prkaa1 (Ampk)Prkaa2Prkab1Prkab2Prkag1Prkag2Prkag3Stk11 (Lkb1)Tsc1Tsc2
mTOR Downstream Effectors
mTORC1 Positive Regulation​
Chuk (Ikbka, Ikka)Eif4bEif4eHif1aIkbkb (IKK2)Rps6Rps6kb1Rps6kb2Tp53 (p53)VegfaVegfbVegfc
mTORC2 Positive Regulation​
Akt1Cdc42Gsk3bHspa4 (Hsp70)IlkMyo1cPrkcaPrkcbPrkcePrkcgRhoaRps6kb1Sgk1
mTORC1 Negative Regulation​
Eif4ebp1Eif4ebp2Ppp2caPpp2r2bPtpaTp53 (p53)Ulk1
Cellular Processes Regulated by mTOR Signaling
Amino Acid Response​
Pik3c3 (Vps34)RragaRragb (QuantiNova Symbol: LOC108348096)RragcRragd
Angiogenesis​
Chuk (Ikbka, Ikka)Ddit4 (Redd1)Ddit4l2 (Redd2)Hif1aIkbkb (IKK2)VegfaVegfbVegfc
Autophagy​
Pik3c3 (Vps34)Ulk1
Cytoskeletal Organization​
Cdc42PrkcaPrkcbPrkcePrkcgRhoa
Growth Factor Response​
Rps6ka4HrasIgf1Igfbp3KrasMapk1 (Erk2)Mapk3 (ERK1)NrasRps6ka1Rps6ka2Rps6ka5
Energy Stress​
Prkaa1 (Ampk)Prkaa2Prkab1Prkab2Prkag1Prkag2Prkag3
Insulin Signaling​
Akt1Akt2Akt3Ins2InsrIrs1Pdpk1Pik3ca (p110-alpha)Pik3cbPik3cdPik3cgPik3r1 (PI3KA)Pik3r2Tp53 (p53)
Lipid Metabolism​
Pld1Pld2
Translation​
Rps6ka4Eif4bEif4eEif4ebp1Eif4ebp2Ppp2caPpp2r2bPtpaRps6Rps6ka1Rps6ka2Rps6ka5Rps6kb1Rps6kb2

Product Resources

Array Layout for PCR Plates
File Size: 138.24 KBLanguage: English
Array Layout for Rotor-Gene Q
File Size: 132.76 KBLanguage: English

Resources

Supplementary Protocols (10)
ABI 7500 & ABI 7500 FAST (Software Version 2.0.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (388KB)
ABI 7900HT (for SDS Software 2.1, 2.3 and 2.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (320KB)
ABI StepOnePlus (for Software Version 2.0) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (77KB)
Bio-Rad CFX96 and CFX384 instrument setup instructions for RT2 Profiler PCR Arrays
PDF (298KB)
Bio-Rad iCycler & iQ Real-Time PCR Systems (for Software Version 3.1) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (249KB)
Eppendorf Mastercycler ep realplex instrument setup instructions for RT2 Profiler PCR Arrays
PDF (554KB)
Stratagene Mx3000P qPCR System real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (259KB)
Rotor-Gene Q real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (175KB)
Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (1MB)
Life Technologies ViiA7 (ViiA 7 Software v1.2) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (511KB)
Brochures & Guides (4)
RT2 Profiler PCR Arrays: Pathway Analysis - (EN)
PDF (1MB)
Total RNA Discovery - (EN)
PDF (255KB)
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Technical Guide to QIAGEN PCR Arrays
PDF (2MB)
JA-PCR-Array---パスウェイ解析
Safety Data Sheets (1)
Safety Data Sheets
Articles (17)
(EN) Control of PPARγ and mTOR in insulin resistance
(EN) miRNA regulation of angiogenesis: new roles for IGF-1R signaling and heparin
(EN) Regulation of BAX mediated apoptosis by BCL2 family members
(EN) Chemokines: manipulators of metastasis
(EN) DNA damage repair — regulation through autophagy
(EN) Silencing cell-cell communication to combat drug toxicity
(EN) Cellular stress, TGF-β, and EMT: what are the true origins of fibrosis?
(EN) The role of inflammation in cancer
(EN) What enhances the inflammatory response: new players and unexpected roles
(EN) Induced pluripotent stem cells: real stem cells?
(EN) Lipid autophagy in obesity and atherosclerosis: chewing the fat
(EN)Metabolic homeostasis: regulation of FOXO by class IIa HDACs
(EN)Regulation of the extracellular matrix by miRNA: impact on cancer metastases
(EN)Inflammation and neurodegenerative disease: a role for the estrogen receptor in suppression of inflammation in the brain
(EN)A good transcription factor gone rogue: the two faces of NRF2 in oxidative stress
(EN)Managing mortality: how cells regulate telomerase activity
(EN) Master Regulators Direct TGF-beta and Smad Signaling in Different Cell Types
Download Files (3)
Housekeeping Gene Data Analysis
XLSX (60KB)
Data analysis file for RT² Profiler PCR Array Housekeeping Genes
Catalog number- 330231
Pathway number- PAXX-000
RNA QC Data Analysis
XLS (484KB)

Data analysis file for RT² ProfilerRT² Profiler™ PCR Array RT2 RNA QC
Catalog number- 330231
Pathway number- PAXX-999

RT2 Profiler PCR Array Data Analysis v3.5 Handbook
PDF (770KB)
For analyzing gene expression data from RT2 Profiler PCR Arrays 
Scientific Posters (1)
Explore the RNA Universe!
PDF (1MB)
Poster for download
Kit Handbooks (2)
(JA) - RT2 Profiler PCR Arrayプロトコールとトラブルシューティング
(425KB)
パスウェイ特異的遺伝子の発現をリアルタイムRT-PCR を用いてプロファイリング
RT2 Profiler PCR Array Handbook
PDF (702KB)
For pathway-focused gene expression profiling using real-time RT-PCR
Performance Data (7)
PCR_Array_4x96_384-Well_Conversion Spreadsheet
XLSX (38KB)
RT2 Profiler Housekeeping Genes PCR Array Data Analysis 1808
XLSX (65KB)
RT2 Profiler PCR Array 384HT Data Analysis Spreadsheet 1808
XLSX (1MB)
RT2 Profiler PCR Array Data Analysis Spreadsheet 1904
XLSX (3MB)
RT2 Profiler RNA QC PCR Array Data Analysis Spreadsheet 1808
XLSX (60KB)
RT2 qPCR Assay Data Analysis 1808 Spreadsheet
XLSX (104KB)
Universal Custom PCR Array Conversion
XLSX (291KB)
Instrument Technical Documents (2)
(EN) - RT2 Profiler PCR Arrays
PDF (431KB)
For pathway-focused gene expression analysis
QIAGEN Service Core - (EN)
PDF (333KB)
For gene expression and genomic analysis
White Papers (2)
RT2 Profiler PCR Arrays: Pathway-focused gene expression profiling with qRT-PCR
PDF (1MB)
RT2 Profiler PCR Array application examples
PDF (563KB)
Gene Expression Analysis (1)
(EN) Wnt Signaling: GSK3 Inhibition Through Sequestration in Multivesicular Bodies
Certificates of Analysis (1)
Certificates of Analysis
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