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Positive results with as little as 25 ng RNA.
High reproducibility among different users.
PCR arrays yield highly accurate results.
A single gene-specific product in every reaction.
Simple procedure.

RT² Profiler™ PCR Array Human Transplant Rejection

GeneGlobe ID: PAHS-166Z | Cat. No.: 330231 | RT2 Profiler PCR Arrays

Product Specification

RT2 Profiler PCR Array

Target List

Acute Rejection - Innate Immunity
Pattern Recognition Receptors (PRRs)
TLR3TLR4TLR9
Cytokines
CCL2CCL4CCL5CCL11CSF2CXCL10IFNGIL10IL12AIL13IL16IL1BIL2IL2RAIL3IL32IL4IL5IL6CXCL8TNF
Other Innate Immunity Genes
ADAM17C3CASP1CASP3CASP8CCR2CCR3CD14CD28CD8AFASFASLGFCGR1AGZMAGZMBMS4A1NFKB1NOS2PRF1PSMB9STAT1STAT6TAP1TNFAIP3TNFSF10
Acute Rejection - Adaptive Immunity
Th1 Markers & Immune Response
CCR5CCR7CD80CTLA4CXCR3IFNGSTAT4
Th2 Markers & Immune Response
CD86IL10IL13IL4IL5IL6
T-Cell Activation
CD80CD86ICAM1IFNG
Cytokines
CCL2CCL4CCL5CSF2CXCL10IFNGIL10IL13IL4IL5IL6TNF
Leukocyte Recruitment
CD40CD40LGICAM1THBS1VCAM1
Chronic Rejection
Pro-Fibrotic
CCL3CCN2IL4IL5IL13
Anti-Fibrotic
BMP7COL1A2IFNGIL10THBS2
Cell Adhesion Molecules
ICAM1ITGA2ITGAEITGAMVCAM1
Cellular Infiltration
BMP7CCR2CD44COL1A2CX3CR1CXCL9CXCL11CXCR4MMP1MMP2MMP7MMP9PECAM1TGFB1TGFB2TGFB3THBS2TIMP1VEGFA

Product Resources

icon_0041_download-s Save Target List
icon_0245_cc_gen_pdf-s Array Layout for PCR Plates
File Size: 141.44 KBLanguage: English
icon_0245_cc_gen_pdf-s Array Layout for Rotor-Gene Q
File Size: 136 KBLanguage: English

Resources

Brochures and Guides (3)
Technical Guide to QIAGEN PCR Arrays
PDF (2.3MB)
Download
RT2 Profiler PCR Arrays: Pathway Analysis - (EN)
PDF (1.4MB)
Download
Total RNA Discovery
PDF (256KB)
Download
Simultaneously profile mRNA, miRNA and lncRNA using a simple, complete workflow
Download Files (3)
RNA QC Data Analysis
XLS (484KB)
Download

Data analysis file for RT² ProfilerRT² Profiler™ PCR Array RT2 RNA QC
Catalog number- 330231
Pathway number- PAXX-999

RT2 Profiler PCR Array Data Analysis v3.5 Handbook
PDF (770.9KB)
Download
For analyzing gene expression data from RT2 Profiler PCR Arrays 
Housekeeping Gene Data Analysis
XLSX (60.1KB)
Download
Data analysis file for RT² Profiler PCR Array Housekeeping Genes
Catalog number- 330231
Pathway number- PAXX-000
Kit Handbooks (2)
RT2 Profiler PCR Array Handbook
PDF (702.8KB)
Download
For pathway-focused gene expression profiling using real-time RT-PCR
JA-RT2-Profiler-PCR-Arrayプロトコールとトラブルシューティング
PDF (425.3KB)
Download
パスウェイ特異的遺伝子の発現をリアルタイムRT-PCR を用いてプロファイリング
Performance Data (7)
PCR_Array_4x96_384-Well_Conversion Spreadsheet
XLSX (38.7KB)
Download
RT2 Profiler RNA QC PCR Array Data Analysis Spreadsheet 1808
XLSX (60.5KB)
Download
RT2 Profiler Housekeeping Genes PCR Array Data Analysis Spreadsheet 1808
XLSX (65.2KB)
Download
Universal Custom PCR Array Conversion
XLSX (291.3KB)
Download
RT2 qPCR Assay Data Analysis 1808
XLSX (105KB)
Download
RT2 Profiler PCR Array Data Analysis Spreadsheet 1904
XLSX (3MB)
Download
RT2 Profiler PCR Array 384HT Data Analysis Spreadsheet 1808
XLSX (1.5MB)
Download
Protocols (10)
Stratagene Mx3000P qPCR System real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (259.3KB)
Download
Bio-Rad CFX96 and CFX384 instrument setup instructions for RT2 Profiler PCR Arrays
PDF (298KB)
Download
ABI 7900HT (for SDS Software 2.1, 2.3 and 2.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (320.7KB)
Download
ABI 7500 & ABI 7500 FAST (Software Version 2.0.4) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (388KB)
Download
ABI StepOnePlus (for Software Version 2.0) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (77.2KB)
Download
Roche LightCycler 480 real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (1.6MB)
Download
Eppendorf Mastercycler ep realplex instrument setup instructions for RT2 Profiler PCR Arrays
PDF (554.4KB)
Download
Rotor-Gene Q real-time PCR run setup instructions for RT2 Profiler PCR Arrays
PDF (175.6KB)
Download
Bio-Rad iCycler & iQ Real-Time PCR Systems (for Software Version 3.1) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (249.7KB)
Download
Life Technologies ViiA7 (ViiA 7 Software v1.2) instrument setup instructions for RT2 Profiler PCR Arrays
PDF (511.3KB)
Download
Scientific Posters (1)
Explore the RNA Universe!
PDF (1MB)
Download
Poster for download
User Manuals (2)
(EN) - RT2 Profiler PCR Arrays
PDF (431.4KB)
Download
For pathway-focused gene expression analysis
QIAGEN Service Core - (EN)
PDF (333.4KB)
Download
For gene expression and genomic analysis
White Papers (2)
Pathway-focused gene expression profiling with qRT-PCR
PDF (1.2MB)
Download
RT2 Profiler PCR Array application examples
PDF (563.3KB)
Download
Safety Data Sheets (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
Certificates of Analysis (1)
Certificates of Analysis
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